A lab technician is running a test on a patient’s sample that is likely infected with salmonella. The technician correctly performs PCR on the sample using primers known to identify salmonella. Then they run the PCR product on an electrophoresis gel. What is the technician likely to see on the gel if the patient does have salmonella?

bands matching the size found in known salmonella samples

bands that do not match the size found in known salmonella samples

no bands

Answers

Answer 1

Answer:

First one

Explanation:

Answer 2

Final answer:

If the patient has salmonella, the lab technician is likely to observe bands matching the size found in known Salmonella samples on the electrophoresis gel, indicating the presence of the bacteria.

Explanation:

Electrophoresis Gel Analysis for Salmonella Prediction

If the patient's sample is indeed infected with Salmonella, the lab technician would expect to see bands matching the size found in known Salmonella samples on the electrophoresis gel after running PCR. This is because PCR specifically amplifies the DNA region targeted by the primers, and if Salmonella DNA is present, its size after amplification should be consistent with known Salmonella DNA fragments. Upon visual confirmation with a gel, distinct bands of the expected size should appear, suggesting a positive identification of the bacteria in the sample.

PCR amplification results in a large number of copies of the targeted DNA segment, usually producing a single, sharp band after gel electrophoresis if the target is present. If no bands are present, this might indicate that the sample did not contain the DNA region targeted by the primers, or there could have been an error in the PCR process. Observing the correct size band confirms the technician's prediction and the presence of the pathogen.


Related Questions

Dr. Tanaka discovered a new virus. The virus has a single strand of a nucleic acid, but she does not know if it is DNA or RNA. After running tests, she concludes that it is DNA. Which of the following would lead her to that conclusion?
A. It contains cytosine.
B. It contains thymine.
C. It contains uracil
D. It contains adenine

Answers

Answer:

Explanation:

I believe it is b

Dr. Tanaka can conclude that the genetic material of this virus is DNA because it contains thymine (Option B).

In DNA, there are four types of nucleotides, each one containing one different nitrogenous base.

These four nitrogenous bases are Adenine, Guanine, Cytosine and Thymine.

Moreover, in RNA, Uracil bases replace Thymine bases.

In conclusion, Dr. Tanaka can conclude that the genetic material of this virus is DNA because it contains thymine (Option B).

Learn more in:

https://brainly.com/question/9086127?referrer=searchResults

Each statement explains a cause that would prevent a plant not to bear seed in its life cycle except:

Answers

Answer:

"The plant will grow to full maturity and produce flowers instead"

1. scientists had identified a pattern in the moths that they thought was important. What was the pattern?
2. The classified identified several factors that could affect populations. One of those factors was abiotic. What are some abiotic factors that you think would be affecting the moth population.​

Answers

Answer:

1. They had predators both at night and through the day, and that they tried to hide and rest on trees but were not very hidden.

2. The chemicals released by the female moths to mate and lay eggs is an important abiotic factor in which affected the population. The more the chemical was released, the more it led to eggs being laid and reproducing.

Explanation:

The moth symbolizes rebirth, change, transformation, resurrection, and the power of regeneration in Native American mythology. In fact, butterflies and moths both hold a significant position in the Native American culture.

How did moths evolve?

Scientists have long attributed these insects' rich variety to their close connections with other organisms. Butterflies, they hypothesized, evolved in tandem with the plants they fed on, and moths developed sophisticated defense mechanisms in response to bats, their main predators.

What is the cycle of a moth?

There are four stages of the moth life cycle: eggs, larvae, pupa/cocoon, and adult. Each stage represents a significant step in a moth's lifespan.

What was causing the change in the moths?

Genetic Changes

Moths passed their color to the next generation. Eggs from light moths developed into light moths and dark moth eggs turned to dark adults. The dark color was caused by a mutation in the DNA of a single moth, and the mutated gene had been passed to all its offspring.

How abiotic factors affect moth populations?

Environmental factors that influence populations are divided into two categories – abiotic and biotic factors. Abiotic factors refer to the non-living physical and chemical elements found in an ecosystem such as rainfall, temperature, pH, sunlight, shelter and day length.

Learn more about moth here: https://brainly.com/question/24203863

#SPJ2

Which DNA or RNA consists of a single copy of a gene

Answers

It is RNA because it is a single strand
rna is single stranded and dna is a double helix

Is a swan a type of duck?

Answers

Yes it is i thinkbxbxb

"The answer is no, a swan is not a type of duck.

Swans and ducks are both members of the Anatidae family, which also includes geese. However, they belong to different genera within this family. Swans are typically placed in the genus Cygnus, while ducks are placed in the genus Anas, among others. Swans are generally larger than ducks and have distinct physical characteristics, such as a longer neck and a different shape of the bill. Additionally, swans and ducks exhibit different behaviors and have different habitats and diets. Therefore, while they are related, a swan is not classified as a type of duck."

The light-independent reactions in plants produce
a. ATP
b. COM
C.
simple sugars
proteins

Answers

Answer:

the answer is ATP

Explanation:

Google


1. Plants produce glucose and oxygen during the process of
absorbs solar radiation and reradiates it to Earth's
2. Earth's
surface.
Key Concepts:

Answers

Answer:

Plants produce oxygen gas and glucose molecule during the process of photosynthesis by absorbing radiation of the sun.

Explanation:

Photosynthesis is a process in which glucose and oxygen are produced by the combination of carbondioxide and water in the presence of sunlight. Carbondioxode is taken in the leaves through stomata and water is absorbed by the plant from the soil. When these two substances combine in the presence of sunlight produces glucose and oxygen.

which factor is most likely to play a role in producing different varieties of cattle? A.necrosis
B.meiosis
C.apoptosis
D.mitosis ​

Answers

Answer:

I think it is Meiosis

Explanation:

In meiosis, 4 or more combinations of the genes are created. This can be graphed using a punnet square.

Final answer:

The most likely factor to produce different varieties of cattle is meiosis, which allows for genetic recombination and diversity through selective breeding. Necrosis and apoptosis, which pertain to cell death, do not contribute to genetic diversity, while mitosis results in identical cells.

Explanation:

The factor most likely to play a role in producing different varieties of cattle is meiosis. Meiosis is the process of cell division in sexually reproducing organisms that results in the production of gametes (sperm and egg cells) with half the number of chromosomes of the parent cell. This reduction leads to genetic diversity when these gametes combine during fertilization. Meiosis allows for genetic recombination and the potential for new traits to emerge, which can be selected for through breeding practices. Therefore, selective breeding, utilizing the genetic diversity produced by meiosis, is instrumental in cultivating different cattle varieties.

On the other hand, necrosis and apoptosis are processes related to cell death. Necrosis is an uncontrolled process often due to injury, while apoptosis is a programmed cell death necessary for development and maintaining cell equilibrium. Mitosis is a process of cellular replication that produces two genetically identical daughter cells and does not contribute to genetic diversity as meiosis does.

Glaciers act as ___ in the water cycle, because they can store water for a long period of time

Answers

Final answer:

Glaciers act as reservoirs in the water cycle, storing water for a long period of time. They play a crucial role in regulating water supply, especially in regions that depend on glacier meltwaters. The retreat of glaciers due to global warming can have significant impacts on local ecosystems and water availability.

Explanation:

Glaciers act as reservoirs in the water cycle because they can store water for a long period of time.

When water from precipitation or melting ice accumulates in glaciers, it gets stored as ice for hundreds or thousands of years. This stored water is released gradually over time, contributing to the overall water cycle. Glaciers play a crucial role in regulating water supply, especially during dry seasons or in regions that depend on glacier meltwaters.

An example of glaciers acting as reservoirs is the Grinnell Glacier in Glacier National Park. The retreat of the glacier due to global warming has led to a loss of summer meltwaters, affecting local ecosystems and water supplies.

Learn more about glaciers acting as reservoirs here:

https://brainly.com/question/34405645

#SPJ12

List the 6 levels of ecological organization – starting with
the term used for an individual living thing, and ending
with the term that represents what the Earth is. How is the
organism you chose in the last lesson a part of each of the
levels? Example: LION- one lion is an individual; A group
of LIONS are a population, etc. (6 pts)

Answers

Answer:

organism, population, communities, ecosystem, and biome; excluding biosphere from the list.....or population, community, ecosystem, and biosphere.

Explanation:

brainliest???

Final answer:

The six levels of ecological organization include organism, population, community, ecosystem, biome, and biosphere. These represent levels from an individual living thing to the broadest scope of all ecosystems on Earth. Any organism you choose will fit into these levels in a similar manner.

Explanation:

The six levels of ecological organization, starting from the smallest unit to the largest, are:

Organism: This is an individual living thing, such as a lion. Population: This refers to a group of the same species that lives in the same area, like a pride of lions in the same savannah. Community: This includes all the different species that live together in a habitat, like lions, zebras, and elephants in the savannah. Ecosystem: This constitutes both the living (biotic) and non-living (abiotic) factors of a particular environment. For instance, a savannah ecosystem would include all its animals, plants, weather, and so forth. Biome: This is a major ecological community spread over a large area, for instance the Savannah biome, which has similar weather, animal, and plant life across multiple continents.Biosphere: This is the part of the earth where life exists, including all biomes and ecosystems. Thus, our lion from the earlier example is part of our global biosphere.

The organism you chose in the previous lesson would fit into these levels in a very similar manner to our lion example.

Learn more about Ecological organization here:

https://brainly.com/question/35172791

#SPJ6

Which shows the correct sequence of steps involved in sexual reproduction?

Answers

Answer:

Meiosis produces sex cells, egg and sperm join. Then zygote forms, new organism develops. Egg and sperm join, zygote forms, meiosis produces sex cells, new organism develops.

Explanation:

Answer:

three stages: Pre-fertilization, Fertilization, and Post-fertilization

Explanation:

Which of the following is NOT a type of tissue found in plants?

O Dermal

O Non-living

O Vascular

O Ground​

Answers

i would say non living because all plants are living yk
Final answer:

Plants have three main types of tissue - dermal, vascular, and ground. 'Non-living' is not considered a type of tissue in plants as plant tissues are primarily formed of living cells.

Explanation:

Plants consist of three main types of tissues, namely: dermal, vascular, and ground tissue. Dermal tissue, which comprises the plant's outer protective coating, vascular tissue, responsible for transporting water, nutrients, and sugars between different parts of the plant, and ground tissue, which includes all tissues not part of the dermal or vascular systems and typically serves a metabolic function. However, 'Non-living' is NOT a type of tissue found in plants. In a physiological sense, it's typically the living cells that form the various plant tissues.

Learn more about Plant Tissues here:

https://brainly.com/question/35053408

#SPJ6

What 4-5 elements from the periodic table are necessary in an ecosphere?

Answers

Answer:

air, water, life (algae, microorganisms and shrimp) and land (gravel and sea fan)

Explanation:

The EcoSphere is a tiny working model of the Earth. It contains the same essential elements that are found on our planet- air, water, life (algae, microorganisms and shrimp) and land (gravel and sea fan).

Which of the following is a reason for discovering a new drug?
A. To confuse patients and physicians
B. To replace a less-affective drug
C. To Create a new disease
D. To duplicate an older drug

Answers

Answer:

B to replace less affective drug.

The reason for discovering a new drug is to replace a less-effective drug.

What is a drug?"It is a chemical substance, which, when administered to a living organism, produces a biological effect."Some of the drugs include: anti-depressant, CNS stimulants, analgesics, etc.Why some drugs become less-effective?As we use the drug for long time it creates a tolerance level, where our body fails to produce a response when administered by such drugs.Over the time, diseases undergo various types of mutation and cannot be treated by using the same drug that was used before. So, a new drugs are being discovered from time to time.

Hence, the correct option is B. To replace a less-affective drug.

To know more about drugs here

https://brainly.com/question/12285237

#SPJ2

Which of the following best describes the use of a non–renewable resource? (2 points)
a. California has several power plants that use heat from the Earth to generate electricity.
b. In the state of Texas, you can find miles of windmills that are used to generate electricity.
c. Oil is burned to heat water into steam in order to turn turbines that generate electricity.
d. The electronic signs along the highway are run by solar power.

PLEASE HELP

Answers

Answer:

c

Explanation:

the answer is c because oil is a non renewable resource due to the time it takes to make

Natural gas is unquestionably a much cleaner non-renewable energy source than coal, but it still contributes to almost a third of the nation's greenhouse gas emissions. Thus, option C is correct.

What is the significance of non-renewable resource?

A resource is nonrenewable if it is consumed more quickly than it can replenish. A nonrenewable resource's supply is limited, which means it is difficult to refill. The Earth itself is used to harvest non-renewable resources.

Nuclear power, natural gas, coal, and oil are examples of nonrenewable energy sources. The fact that we now rely on these resources to provide the majority of our energy needs makes the fact that they cannot be replenished after they are depleted a serious issue for humanity.

Therefore, oil is burned to heat water into steam in order to turn turbines that generate electricity, best describes the use of a non-renewable resource.

Learn more about non-renewable resource here:

https://brainly.com/question/14214221

#SPJ2

Why is surface tension important

Answers

Answer:

Surface tension determines the efficiency of detergent formulation. The high surface tension of water makes it a relatively poor cleaning detergent. By increasing the temperature of water, the cleaning efficiency increases slightly as surface tension decreases.

Explanation:

Which is a possible negative result of using renewable resources?

Answers

Loss of animals is a possible negative result

Answer:

Explanation:

h

What ability have many cave fishes lost

Answers

Answer:

most likely sight

Explanation: it is very dark in caves anyways, and their pupils would need to adjust to light, and of they have been living in a cave for a while, they would have adapted larger pupils to take in any possible light.

Answer:

the ablility to see

Explanation:

in figure 13-1 between which nucleotides is the DNA cut?
(A) ADENINE AND THYMINE
(B) CYTOSINE AND GUANINE
(C) THYMINE AND CYTOSINE
(D) ADENINE AND GUANINE

Answers

Answer:

d

Explanation:

because i studied that this will be the ans

I’m not sure because I don’t know what figure you’re referring to. sorry. :(

The
cycle is not a nutrient cycle

Answers

Answer:water cycle

Explanation:

Final answer:

Biogeochemical cycles, such as the carbon, nitrogen, and phosphorus cycles, play a crucial role in ensuring the continuous availability of essential nutrients for all living organisms, including their recycling through the ecosystem and non-living world.

Explanation:

Biogeochemical Cycles

Biogeochemical cycles refer to the processes responsible for the cycling of mineral nutrients through ecosystems and the non-living world. These cycles ensure that essential nutrients, which are vital for the growth and survival of all organisms, are reused and recycled within the environment. Among the significant nutrient cycles are the carbon cycle, nitrogen cycle, and phosphorus cycle. Every living organism relies on these cycles for nutrients that are crucial for their growth and development.

For instance, the carbon cycle involves plants taking in carbon dioxide during photosynthesis and releasing oxygen. The nitrogen and phosphorus cycles are critical in providing mineral nutrients to plants from the soil. These nutrients then flow through the ecosystem, supporting various forms of life, and are eventually returned to the soil and atmosphere where the cycle begins again.

Disruptions in nutrient cycles can lead to environmental issues such as nutrient leaching and a decline in soil fertility, impacting both plant and animal life. The condition of natural vegetation cover is essential in maintaining these cycles, as plants play a significant role in the absorption and release of nutrients.

A biologist wishes to take a useful gene found in penguins and introduce it into some bacterial cells. Her experimental procedure is given below.

Use a restriction enzyme to cut a gene out of the penguin DNA.
Use a restriction enzyme to cut open a plasmid.
Treat the plasmid with DNA ligase.
Treat the plasmid with DNA polymerase.
Introduce the plasmid into bacterial cells.

A step is missing. Where should it go in this procedure?

“Mix the desired gene and plasmid together” should go after step 5.
“Mix the desired gene and plasmid together” should go between steps 3 and 4.
“Mix the desired gene and plasmid together” should be the first step.
“Mix the desired gene and plasmid together” should go between steps 2 and 3.

Answers

Answer:

The last one IM like 80% sure its the answer

Explanation:

Final answer:

The missing step, "Mix the desired gene and plasmid together," should be placed between steps 2 and 3 in the procedure, after the plasmid has been cut and before it is treated with DNA ligase.

Explanation:

The step "Mix the desired gene and plasmid together" should take place after the plasmid has been cut with a restriction enzyme and before it is treated with DNA ligase. The correct sequence of steps in the procedure would be:

Use a restriction enzyme to cut a gene out of the penguin's DNA.Use a restriction enzyme to cut open a plasmid.Mix the desired gene and plasmid together.Treat the plasmid with DNA ligase to join the gene of interest to the plasmid DNA.Treat the plasmid with DNA polymerase if necessary.Introduce the plasmid into bacterial cells.

This sequence ensures that after the plasmid is opened, the gene of interest is present for ligation, creating a recombinant plasmid to be introduced into the bacteria for gene expression.

America has 36 total chromosomes in each body cell. After meerkat cells undergo the process of meiosis each gamete will have how many chromosomes

Answers

Answer: 18 chromosomes

Explanation: Gametes have half the number of chromosomes as normal body cells. 36/2=18.

A plant takes in carbon dioxide (CO2) from the atmosphere and uses it to make glucose (C6H12O6) through photosynthesis. Later a deer eats the leaves of the plant obtaining the plant’s glucose. After respiration, the deer exhales carbon dioxide (CO2) back into the atmosphere. This is an example of _________________.

Answers

Final answer:

The process described is the carbon cycle, which involves the movement of carbon between different parts of the ecosystem through the processes of photosynthesis and respiration.

Explanation:

A plant takes in carbon dioxide ([tex]CO_{2}[/tex]) from the atmosphere and uses it to make glucose ([tex]C_{6}H_{12}O_{6}[/tex]) through photosynthesis. Later a deer eats the leaves of the plant obtaining the plant's glucose. After respiration, the deer exhales carbon dioxide ([tex]CO_{2}[/tex]) back into the atmosphere. This is an example of the carbon cycle.

The carbon cycle is a fundamental biogeochemical cycle, which involves the movement of carbon between the atmosphere, biosphere, oceans, and geosphere. During photosynthesis, plants use sunlight to convert atmospheric [tex]CO_{2}[/tex] and water into glucose ([tex]C_{6}H_{12}O_{6}[/tex]) and oxygen ([tex]O_{2}[/tex]). This glucose is then used by the plant for growth and energy. When a heterotroph, such as a deer, eats the plant, it obtains energy by breaking down the glucose during respiration. The respiration equation is: [tex]C_{6}H_{12}O_{6}[/tex] + 6 [tex]O_{2}[/tex] → 6 [tex]CO_{2}[/tex] + 6 [tex]H_{2}O[/tex] + Energy released.

In this process, as glucose is broken down, carbon is returned to the atmosphere in the form of [tex]CO_{2}[/tex], thus completing the cycle. This illustrates how carbon is continuously cycled within the ecosystem through the processes of photosynthesis and respiration.

2)Through the action of osteoclasts,

Answers

Answer:

Osteoclasts are the type of cells which are responsible for the absorption of calcium from bones.

Explanation:

Osteoclasts is special type of cells which is released by parathyroid gland when the blood have low levels of calcium. Osteoclasts absorb calcium from the bones and add this calcium into the blood to increase the calcium level. If calcium is present in very high amount so this calcium is stored in the bones of the body and extracted when needed by the body.

How are rocks that contain oil
similar to a sponge?

Answers

Answer: Most oil deposits are deep underground. There, the oil is usually found within tiny pores, or holes, in rocks such as sandstone and limestone. Rocks containing oil are a little bit like a sponge that has soaked up water.

Llamas have sexual reproduction. Some types of llamas have 74 chromosomes. That is how many chromosomes are in their somatic (body) cells. How many chromosomes would be found in their gametes?

Answers

Answer: 37 chromosomes.

Explanation: As is typical of mammals, llamas are diploid organisms, meaning that the number of chromosomes in each of their somatic cells is exactly twice of that found in the gametes (the so-called haploid number, referred to as "n"). If a particular species has 74 chromosomes in its somatic cells, we should expect to find 74/2=37 chromosomes in each gamete.

Sexual reproduction occurs as a result of the fertilization of the gametic counterparts of two different sexes (males and females).

Some types of llamas have 74 chromosomes (diploid). The number of chromosomes that would be found in their body cells would be 32 single chromosomes (haploid cells).

Organisms undergo growth division process of mitosis and meiosis.Mitosis occurs in the body cells producing the exact number of diploid cells from parent cell. Meiosis is a reductional division that reduces the number of chromosomes in the gamete cells by half.This is to ensure that the fertilization of the egg gamete (haploid) and the sperm cell (haploid) yields a diploid zygote.

Learn more: https://brainly.com/question/17179690

. In which phylum do organs and organ systems first appear?
a. Annelida
b. Hemichordata
c. Nematoda
d. Platyhelminthes​

Answers

Answer:

The answer is d. Platyhelminthes

What proportion of tropical storms turn into hurricanes?​

Answers

10%
in the atlantic ocean, above the equator, there are typically 100 storms during hurricane season that begins this way. roughly 10% of these storms become tropical storms and only 6% produce winds that are strong enough to be classified as hurricanes.

Individuals with characteristics that are poorly suited to the environment are _______ likely to survive and reproduce. *

Answers

Answer:

less

Explanation:

not, less,

anything that means the same as that. bc if they're less suited. they die.

Deforestation is a process in which trees and other vegetation are removed from a section of land. What statement best describes the long-term consequences of deforestation on the carbon cycle?
A.
More carbon will be released by carbon sources, so the level of atmospheric carbon may decrease.
B.
Less carbon will be stored in carbon sinks, so the level of atmospheric carbon may increase.
C.
Less carbon will be released by carbon sources, so the level of atmospheric carbon may increase.
D.
More carbon will be stored in carbon sinks, so the level of atmospheric carbon may decrease.

Answers

B

hope this helps you good luck
Other Questions
find the missing angle..? x=? Tracy's uncle came over for dinner from France.What confusion is caused by the misplaced modifier in the sentence on the left?It seems like Tracy is eating the dinner.It seems like Tracy went to France.It seems like Tracys uncle missed dinner.It seems like the dinner was from France In triangle MNP, what is the measure of angle N? IntroductionThis book will look at homeschooling from two perspectives. It will show how homeschooling affects both teachers and students. I will show that there are both pros and cons to homeschooling.Though it is not for everyone, there are ways that homeschooling can support certain types of students. Students can work at their own pace and explore subject areas that interest them in depth.However, they may also feel separated from peers. In addition, homeschooling is a lot of work for parents and guardians. It requires a lot of resources.The book will also attempt to answer questions about homeschooling. For example, what is it like to learn outside of a traditional classroom? What does a student gain? And what might they lose? What do classroom teachers think of homeschooling? And how are parents and guardians affected? These and other difficult questions will be considered throughout the text.Read the passage, then write a short paragraph analyzing how the text feature and the organizational pattern help the reader. Follow these steps:Describe the purpose of the text feature.Identify the organizational pattern.Analyze how the text feature and the organizational pattern combine to helpRead the passage, then write a short paragraph analyzing how the text feature and the organizational pattern help the reader. Follow these steps:Describe the purpose of the text feature.Identify the organizational pattern.Analyze how the text feature and the organizational pattern combine to help readers. PLZ HELP FOR 100 pt readers. 2/3 is greater than, less than it equal to 5/8 What are the roots of the equation 16x2 - 16x + 5 = 0 in simplest a + bi form? How much energy is needed to vaporize 75.0 g of diethyl ether (c4h10o) at its boiling point (34.6c), given that hvap of diethyl ether = 26.5 kj/mol? Who were the KKK, what was their purpose? The W3C advisory committee must review every proposal for a(n) ______ made up of representatives of the W3C membership group, invite experts, and W3C team members to work on the ________, which may involve writing one or more ______. These go through an extensive review process that includes Requests For Comments (RFC). At any point in the process, a(n) _______ may be terminated. There are 4 green crayons, 6 yellow crayons and 3 blue crayons. P(greenthen blue) without replacement. * how many hydrogen atoms are in 35.0 g of hydrogen gas Which of the following statements is true regarding a representative sample?A.It is at least 1/2 of the total population.B.It focuses on a specific group within the population.C.It is a group that is fairly proportional to the population. As Marie rounded the corner, she saw that people were already boarding her bus. Worried that she would miss her ride to campus, she sprinted to the bus stop. In what order were skeletal muscles recruited during her sprint? During its first year of operations, White Company bills credit customers $15,100 for services rendered. During the year, White receives $10,500 from all customers, $2,500 of which is received from cash customers. Required: What amount of revenue should be shown on the income statement for the year Are these shapes similar? Which of the below rhetorical devices does the advertisement use in its firstsentence?AAstereotypingstereotypingB hyperboleBhyperboleCanecdote What is the range of 16,17,17,18,18,18,19,19,19,19,20,20,20,21,21,22,22,24,25,25 What is the height of our classroom door is it a statistical or non statistical question Find the circumference of a circle that has a diameter of 42 m. Find the area to this please