Brainstorm how you could use recombinant DNA technology to make normal E. coli bacterial cells glow green.

Answers

Answer 1

Answer:

In order to make E. coli bacterial cells glow green, we need to insert a gene that can be translated into the green fluorescent protein. This gene is well-known as gfp and can be inserted either in the DNA or plasmid of the bacteria. The genome insertion procedure would follow the conventional method where the DNA will be first cut using restriction enzymes and then would be ligated. This approach has been extensively applied to test the fate of bacteria in the environmental systems.

A schematic diagram on insertion of gfp in the plasmid of bacteria is shown in the figure. The left side diagram shows that gfp is inserted and right side figure shows glowing bacteria in the presence of UV.

Brainstorm How You Could Use Recombinant DNA Technology To Make Normal E. Coli Bacterial Cells Glow Green.
Answer 2

The usage of recombinant DNA technology to make normal E. coli bacterial cells glow green should be explained below.

Usage of recombinant DNA technology:

For making the E. coli bacterial cells glow green, here we required to insert the gene that should be translated into the green fluorescent protein. This gene should be called as the GFP also could be inserted either in the form of DNA or bacteria plasmid.

The insertion procedure should followed the conventional method in which the DNA should be fist cut via usage the restriction enzymes.

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Related Questions

FIRST PERSON CORRECT WILL BE MARKED BRAINLIEST
Which of the following is not a reason the human population is growing exponentially?
A. modern medicines prevent many childhood diseases

B. advancements in technology mean people can move if they deplete resources in an area.

C. food production has increased as a result of advancements in science and technology

D. infectious diseases can spread in crowded areas

Answers

Answer:

D

Explanation:

D is a reason population would fall

Answer:

Option (d). Infectious diseases can spread in crowded areas is not a reason the human population is growing exponentially.

What are the causes for human population?

Good healthcareIncrease in birthrateFood securityLack of educationCultural influenceLack of future planning

Why other options are incorrect?

The other options are incorrect because they are the reason behind human population rather than option (d) which is not the reason.Thus, option (d) is correct answer.

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The graph below shows the population of mice in an ecosystem where the mice are not allowed to enter or leave.
Which best describes the population at point B?
A.The death rate is higher than the birthrate.
B.The birthrate is higher than the death rate
C.It has reached its carrying capacity
D.The population is decreasing

Answers

It’s birthrate is higher than its death rate

Natality, mortality, and growth in populations that exhibit a logistic growth model depend on the density. Option B.The birthrate is higher than the death rate

What is logistoc growth?

Populations exhibiting a logistic growth model depend on density.

Natality and mortality depend on the population size, meaning that there is no independence between population growth and population density.

When a population grows in a limited space, density rises gradually and eventually affects the multiplication rate. The population per capita growth rate decreases as population size increases. The population reaches a maximum point known as the carrying capacity, K.

The carrying capacity might be affected by different factors, known as limiting factors, which might be a result of the population density (for example, competition) or might be density-independent.

What is carrying capacity?

Carrying capacity refers to the maximum point at which the environment can support a growing population.

K is a constant. It coincides with the population size when natality rate and mortality rate are equal to each other. This is the equilibrium point.

• If the population size, N, is inferior to K ⇒ the population still grows.

• When N approximates to K, the population's growth speed decreases.

• When N=K, the population reaches equilibrium,

• When N is superior to K (N>K), the population must decrease in size because there are not enough resources to maintain that size.

In the exposed example, option B best describes the population at point B. The birth rate is higher than the death rate. At this point the population is experiencing exponential increase in size.

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seasons why do we have them

Answers

We have seasons because the earth is tilted as it makes its way around the sun. So its always pointed in one way at the sun, causing different asmophere as we go around the sun.

Which of the following is an example of conduction A a dog bowl of water warms up out in the sun B leftovers become warm when reheated in the microwave. C campers warm up while sitting by a campfire D a metal spoon becomes warm when placed in a cup of hot tea

Answers

Answer:

Which of the following is an example of conduction?

a metal spoon becomes warm when placed in a cup of hot tea

Explanation:

      Answer: D, a metal spoon becomes warm when placed in a cup of hot tea.                                                                                                                                                                                

      Explanation: Conduction is the transfer of energy through matter from particle to particle. It is the transfer and distribution of heat energy from atom to atom within a substance. For example, a spoon in a cup of hot soup becomes warmer because the heat from the soup is conducted along the spoon.

what is a photosynthesis

Answers

Answer:

the process by which green plants and some other organisms use sunlight to synthesize foods from carbon dioxide and water. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a byproduct.

Answer:

the process by which green plants and some other organisms use sunlight to synthesize foods from carbon dioxide and water. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a byproduct.

Describe what scientists mean when they refer to an ecological community such as that shared by the leopards and lions.

Answers

Answer:

An ecological community can be described as all the different species of organisms i.e all the different plants and animals, living in a particular habitat at a specified time.

An ecological community will consist of:

Predators: Organisms which feed on other organisms.

Preys: The organisms eaten by predators.

Competitors: Different organisms feeding on the same prey and hence, competing for it. For example the leopards and the lions in a community.

Final answer:

An ecological community refers to interacting populations of different species in a specific habitat. Ecologists study these communities to understand species interactions and competition for resources. Factors like habitat conditions and available species determine which organisms can exist within a particular community.

Explanation:

When scientists refer to an ecological community, they are describing a group of interacting populations of different species occupying a given habitat. For instance, the lion and leopard populations living within the same geographic region. The concept of an ecological community also entails the diversity of these species in terms of their number and relative abundance.

Ecologists study these communities to understand how these species interact with each other and compete for the same resources. These species interactions, include predation, herbivory, competition, and pollination, can regulate population sizes and affect other ecological processes impacting diversity.

Within this framework, numerous factors influence the make-up and dynamics of a community, such as the habitat's latitude, rainfall, topography, and the range of species available. These variables are crucial as they determine which organisms can thrive within a particular area.

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Which of these may be a matter of serious concern for an ENVIRONMENTALIST?
1.increase in tigers which is a threat to herbivourous animals
2.involment of more tribal people in the conservation than proffesionals
3.commercial extraction of timber and other forest produce to meet urban demands
4.exploitation of migrated labourers by contractors in a construction site

Answers

Answer:

the answer is #3

Explanation:

How are animals and plants different? How are they the same? Be specific.

Answers

plants give off oxygen and take in carbon dioxide and animals take in oxygen and give off carbon dioxide.

A mutation near the middle of a gene changes the DNA to the following nucleotides: ATT. What
kind of mutation occurred in this situation?
A) missense mutation
B) nonsense mutation
C) silent mutation
D) none of these

Answers

Answer:

A mutation near the middle of a gene changes the DNA to the following nucleotides: ATT. What  kind of mutation occurred in this situation?

missense mutation

Explanation:

Remora fish get free food by hanging out with sharks. Sharks are not affected by
the remora. Which type of symbiotic relationship is this? *

Answers

Answer:

This is a symbiotic relationship of commensalism

Explanation:

Commensalism is when one animal in a symbiotic relationship gets something out of the relationship without harming or benefiting the other. If the fish were to be helping the shark, it would be mutualism (since they both get something out of the relationship). If the fish were to be harming the shark it would be a parasitic relationship. Since the fish is neither hurting or aiding the shark, it is a commensalistic symbiotic relationship!

A major problem with the use of electrical energy is that _____ A. It is not easily stored b. There are very few ways to create it c. It cannot be converted into other forms of energy d. If cannot be used to do work

Answers

Answer:

B

Explanation:

how a 300-N force can combine with a 100-N force on sled

Answers

Answer:

If a 300-N force pushes you forward on a sled, and a 100-N force pushes you back (i.e. the wind), the resultant net force is still 200-N forward

Explanation:

What are reactants in biology

Answers

Answer:

A substance that undergoes a change during a reaction.

Explanation:

Answer: A substance that undergoes a change during a reaction.

Each category represents one of the functions of proteins. Match each example with the appropriate function.

Answers

Answer:

Maintaining homeostasis: Insulin controls the concentration of sugar in the bloodProviding structure: Collagen makes skin strong yet flexibleRegulating chemical reactions: Pepsin works in the stomach to speed up the breakdown of food.Fighting disease: IgA prevents the buildup of viruses and bacteria in the gut.

Explanation:

Homeostasis is the maintenance of internal environment of the cell with respect to the external environment. In the first statement, insulin is controlling the sugar level in the blood cells. Thus it is an example of homeostasis.

Collagen is an abundantly found protein in the mammals. It provides structural support to the connective tissues, bones, and muscles.

Pepsin is a digestive enzyme that breaks down proteins into respective amino acids. Our food normally have proteins which are thus degraded by the action of pepsin enzyme.

Immunoglobulin A or "IgA" is an antibody that play a vital role in immune/defense system. More precisely, it provides protection against viral and bacterial infections, e.g. influenza virus infection.

Final answer:

Proteins in a cell can serve multiple functions, such as acting as enzymes to accelerate internal chemical reactions, acting as hormones that send signals across the body, functioning as antibodies to battle foreign bodies in the immune system, or carrying essential molecules like oxygen as done by hemoglobin.

Explanation:

Without the specific examples and options for their functional categories, it's challenging to provide precise solutions to your question efficiently. However, I can help you understand the main functions that proteins can have in a cell based on standard biology knowledge. First, there are enzymes that act as catalysts to speed up chemical reactions within cells. Second, proteins can be hormones that transmit signals from one part of the body to another. Third,  antibodies are proteins helping in immune responses by identifying and neutralizing foreign bodies like bacteria and viruses. Lastly, proteins, such as hemoglobin, can transport molecules like oxygen throughout the body.

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Black bears eat both plants and small animals in their ecosystem. Which of
the following terms best describes a black bear?
A. Producer
OB. Herbivore
O
C. Omnivore
D. Carnivore

Answers

Answer:

The answer is C. Omnivore

Explanation:

An omnivore is an animal that eats both plants and animals

C. omnivore I Just done this Question.

Why is the disposal of nuclear waste a greater problem than the disposal of the trash that you and you family need to get rid of

Answers

Answer:

nuclear waste has radiation, which is harmful to humans, so nuclear waste has to be stored in special facilities. Nuclear waste takes a lot longer than regular waste to decompose too.

Explanation:

GIVING BRAINLIEST!!!
scientists suggest that galaxies are moving away from earth due to
a - dark energy
b - doppler shift
c - gravity
d - solar energy

Answers

Its dark enegry

Dark energy is like a rapid ageing proces to the earth, its not that the galxaies are moving away from us than that the earth is get bigger.

It’s answer A hope this help you

8. Which of the following is a requirement for exponential growth of a
population?
O A. There's competition from other organisms.
O B. There's a finite number of resources.
O C. There are no limiting factors.
O D. The population is in a climax community.

Answers

It would have to be B. because for something to grow it needs resources such as food and water.

Answer:

C. There are no limiting factors.

Explanation:

in order to replicate itself, a virus must first do what

Answers

Viruses must first penetrate and enter the cell before viral replication can occur.

What is the process of succession?
O
A. When a community is replaced by another community
O
B. When one disturbance replaces another disturbance
O
C. When a species changes from one niche to another niche
O
D. When an ecosystem changes from one biome to another biome



Answer : when a community’s replaced by another community

Answers

Answer:

Change in the species structure of an ecological community over time. (A)

Explanation: Google

AAnswer:A

Explanation:

Charlie lifts a box with a force of 500 N and sets it on a table top 1.2 m above its starting position. Lauren pushes an identical box up a 5 m ramp from the floor to the top of the same table. Which person did more work? Please explain.

Answers

Answer:

Explanation:

Workdone is the force applied to move a body in a specified direction.  

  Work done  = Force x distance

For first box;

          Work done  = 500 x 1.2  = 600J

For the second box = 500 x 5  = 2500J

Lauren did more work compared to Charlie because he move the box through a larger height which is the distance in this scenario.

Final answer:

Charlie and Lauren both elevate boxes to the same height, but assuming no friction and only addressing vertical displacement, Charlie does 600 J of work, while Lauren would do more due to the 5 m displacement along the ramp, resulting in 2500 J if the full force is applied over the entire distance.

Explanation:

The student is asking about the concept of work in physics, which is calculated as the product of force and displacement in the direction of the force. In this scenario, both Charlie and Lauren are performing work to elevate boxes to the same height, but the question is which person did more work. The key concept is that work is force times the displacement in the direction of the force. So, for Charlie, who lifts the box directly upward for a displacement of 1.2 m with a force of 500 N, the work done can be calculated by the formula:

W = F × d = 500 N × 1.2 m = 600 J (joules)

Lauren's situation is slightly more complex because she is pushing the box up a ramp. Nevertheless, if we assume no friction and only account for the vertical displacement, which is the same height that Charlie lifted the box, the work done by both would be the same because they displaced the box the same vertical distance against gravity. However, if we consider the actual distance moved along the ramp and the force applied, Lauren would exert the force over a longer displacement of 5 m:

W = F × d = 500 N × 5 m = 2500 J

Therefore, assuming that Lauren applies the force through the entire length of the ramp, she would indeed do more work due to the longer displacement even though the vertical change in both situations is the same. This indicates that not all of the 500 N force from Lauren is directed towards raising the height of the box; some of it is directed along the ramp to overcome gravity and any potential friction.

The leaves and petals of dicots are found in multiples of____ or____.
A.four; five
B.five; six

Answers

A. four;five is the answere

Answer:

a

Explanation:

usatestprep

Which list contains only abiotic conditions that might be found in a pond ecosystem? *
1 point
Temperature of the water, green plant populations, dissolved minerals in the water
Temperature of the water, dissolved oxygen in the water, dissolved minerals in the water
Bacteria, dissolved minerals in the water, temperature of the water
Dissolved oxygen in the water, fish populations, insect populations

Answers

Answer:

Temperature of the water, dissolved oxygen in the water, dissolved minerals in the water

Explanation:

Abiotic conditions strictly comprises on non-living elements. In the first statement, green plant population is mentioned which is a biotic factor.

In the 3rd statement, bacteria are listed which are biotic entities. Their presence suggest the involvement of biogeochemical cycles in the lake, thus, highlighting both biotic and non-biotic conditions.

In the 4th statement, fish and insect population is mentioned which are also biological entities.

Only second statement is correct because it essentially talks about the abiotic conditions such as temperature, dissolved oxygen and dissolved minerals. Although these parameters could affect the life inside the pond (which cannot be exculded in reality); nevertheless, the need of statement is to identify only those parameters which are abiotic.

Which term best describes the community shown in the photograph?

Answers

Answer: large population for Ap!x

What idea did Hardy and Weinberg disprove?

A. There are seven conditions for equilibrium.

B. Dominant alleles become more common in each generation.

C. The gene pool for a population frequently changes.

D. Allele frequency is stable between generations.

Answers

B: Dominant alleles become more common in each generation

According to Hardy-Weinberg the allelic frequencies remain the same through generations. Option is B. dominant alleles become more common in each generation.

What are the Hardy-Weinberg theory?

The Hardy-Weinberg equilibrium is a theory that states that when a population is in equilibrium, its allelic and genotypic frequencies remain the same through generations.

No evolutive forces are acting on these populations (natural selection, genetic flow, genetic drift, mutation), there are random matings, no superposed generations, and the population size is infinite.

The correct option is B. They disproved that dominant alleles become more common in each generation.

This can not be true since allelic frequencies remain the same through generations.

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In flies, long wings are a dominant trait, and short wings are a recessive trait. Medium wings are the heterozygous trait. Based on this information, if a homozygous long-winged fly is crossed with another a heterozygous fly, their offspring will have which percentages for long, medium, and short wings? Assume random chromosome segregation.

Answers

Answer:

there is a 100% chance it will contain the recessive allele and a 50% chance it will have the recessive trait  

I tried to attach a document I made of a punnet square to help further explain  

Explanation:

A raccoon consumes 500 calories of food. 50 of those
calories are converted to biomass. In other words:

Answers

Final answer:

The subject of this question is the conversion of calories to biomass in a raccoon's diet. This process is known as energy transfer or energy flow in an ecosystem.

Explanation:

In biology, the subject of this question is the conversion of calories to biomass in a raccoon's diet. This process is known as energy transfer or energy flow in an ecosystem.



Calories are a unit of energy, and when a raccoon consumes food, it uses some of that energy for its own metabolic processes and converts the remaining energy into biomass, which is the living tissue of the raccoon's body.



In this case, if the raccoon consumes 500 calories and 50 calories are converted to biomass, the remaining 450 calories are used by the raccoon for its bodily functions.

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According to the question, out of the 500 calories consumed by the raccoon, only 50 are converted into biomass. In simpler terms, for every 500 calories of food it eats, the raccoon uses 50 calories to build up its body mass.

When the raccoon consumes 500 calories of food, it undergoes various metabolic processes to utilize this energy. Out of these 500 calories, only 50 calories are converted into biomass. This conversion involves synthesizing new molecules and tissues, such as muscle, fat, and organs, contributing to the raccoon's growth and maintenance.Metabolic rate is the amount of energy expended by an animal over a specific time. In the case of the raccoon, only a small portion of the consumed calories is converted into biomass. The remaining 450 calories are likely expended as energy to power the raccoon's daily activities, including locomotion, thermoregulation, and cellular functions like respiration and digestion. These calories are transformed into kinetic energy, heat, and other forms of metabolic energy to sustain the raccoon's life processes

Nitrogen: A element only found solely in storage sites of plants and soil
Check all the statements that are true in regards to the carbon cycle*
10 points
Photosynthesis is the process that releases oxygen into the atmosphere
O
Carbon dioxide is taken in as a reactant throughout the process of cellular respiration
The ocean, living things, rocks and soil all act as reservoirs for carbon
Only animals go through the process of respiration
O
Gas, oil and coal are fossil fuels that are primarily composed of carbon

Answers

Answer:

Photosynthesis is a process that releases for oxygen into the atmosphere.

The ocean, living things, rocks and soil are all reservoir of carbon.

Gas, oil and coal are fossil fuels that are primarily composed of carbon.

Explanation:

Carbon cycle is a biogeochemical cycle in which carbon compounds are converted and released in the environment. It involved processes where carbondioxide is used with water and light energy in the process of photosynthesis to produce food and release oxygen to the atmosphere. It also involve the process of respiration where carbondioxide is released to the atmosphere after taking in oxygen. It is also involve a process that release carbon when burning of fossil fuels and decay of dead organisms.

Carbo is considered the major composition of living organisms sand it's also the components of biological molecules.

Land plants are divided into two main groups. They are_____and_____.
A.vascular; nonvascular
B.flowering; nonflowering

Answers

Answer:

Land plants are divided into two main groups. They are vascular and non vascular

The answer is a but I’m Not really sure

5 pieces of evidence that supports climate change

Answers

Answer:

1.Climate change is real and man-made, and there is overwhelming scientific consensus that this is true

2.All major climate change reports are thoroughly researched and based on the most accurate, up-to-date science

3.Climate change studies are transparent

4.Addressing climate change will strengthen the economy

5.Federal climate change reports are credible because they are written by scientists, not politicians

Explanation:

I just use these on a test and got it right

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(3) Pennsylvania women also worked for many reforms, including women's rights, during the 1700s and1800s; although rights for women changed little during this time, their efforts laid the groundwork for future reforms.Which is the most effective way to vary the length of the sentences in the passage?Combine sentences 1 and 2.Change one of the sentences into a phrase.Divide long sentences into two or more shorter ones.Add a subordinate clause to sentence 1.3 of 10-SubmitUS$ 10:53 What is the length of Line segment B C? Round to the nearest tenth.Triangle A B C is shown. Angle B C A is a right angle and angle C A B is 65 degrees. The length of hypotenuse B A is 16 centimeters and the length of B C is x.6.8 cm7.5 cm14.5 cm17.7 Choose the best translation for the following sentence.Give me that! Dalo eso! Me da eso!Dame eso! Danos eso! write a paragraph about ocean currents Rustys favorite meal consists of a corn dog, tater wedges, and a fudge sickle. 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