Briefly explain the steps in the process of removing the human gene and inserting it into plasmid DNA

Answers

Answer 1
)Insert gene of interest into plasmid
2)recombinant DNA is placed back into bacteria
3)bacteria divides
4)gene is now expressed in bacteria and product can be harvested
Answer 2

Answer:

Plasmids are extracted from the bacteria

The plasmids are spliced at specific regions using restriction enzymes.

The cut plasmids are incubated with the novel gene and DNA ligase

Plasmids that will have incorporated the new gene are transformed back into the bacteria

Explanation:

just took the exam and got 50/50


Related Questions

Which of the following is an example of a homozygous dominant allele? wr Rr RR Rw

Answers

Homozygous dominant allele is RR

An example of a purebred dominant allele is RR.

What do you mean by Allele?

An allele may be defined as one or two or more alternate forms of a gene.

An example of a purebred recessive allele is rr. While the allele Rr represents the heterozygous dominant condition.

Therefore, an example of a purebred dominant allele is RR.

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what makes petroleum a nonrenewable resource

Answers

A non-renewable resource ... petroleum, natural gas) and ... It shows that efficient exploitation of a nonrenewable and nonaugmentable resource would, ...

A nonrenewable resource is a resource of economic value that cannot be ... nonrenewable resources are formed from ... since its radioactive nature makes it ...

Final answer:

Petroleum is a nonrenewable resource because it takes millions of years to form and cannot be replaced as fast as it is consumed. Our current usage rates will deplete petroleum reserves in decades, emphasizing the need for alternative energy sources.

Explanation:

Petroleum is considered a nonrenewable resource because it forms under the Earth's surface over millions of years from the decomposed organic materials such as dead plants and animals. This long formation process means that once we have extracted and used petroleum, it cannot be replenished on a human time scale. At our current rates of consumption, petroleum reserves are depleting rapidly, and it is estimated that they will be significantly diminished within decades. Therefore, the crux of the matter is that the rate of consumption far exceeds the rate of natural formation, leading to a finite availability of petroleum.

Nonrenewable resources, like fossil fuels, also pose environmental challenges when burned, such as releasing pollutants and greenhouse gases that contribute to climate change. As a result, there is a growing emphasis on developing sustainable alternative energy sources to reduce our reliance on nonrenewable resources and mitigate environmental impacts.

What happens during the process of translation?

Answers

The entire process is called gene expression. In translation, messenger RNA (mRNA) is decoded in a ribosome, outside the nucleus, to produce a specific amino acid chain, or polypeptide. The polypeptide later folds into an active protein and performs its functions in the cell.

During the process of translation, the cell reads information from the messenger RNA and then uses this to form proteins.

What is translation?

Translation is one of the many transformations that proteins have to undergo. In this process, the proteins read information from the messenger RNA codons, and from the information they get, they form proteins.

The chains that are formed during the process of translation result in polypeptide chains that form proteins.

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The Fugate family, also known as the "Blue People of Kentucky" have a condition known as methemoglobinemia which prevents hemoglobin from carrying oxygen and makes it difficult for unaffected hemoglobin to release oxygen to body tissues. These individuals had a blue complexion, but were otherwise healthy, with many living into their 80's or 90's. These individuals generally have methemoglobin levels of 10-20% of total hemoglobin. How does genetic drift account for the consistency of this level of methemoglobin?

Answers

The genetic drift account for the consistency of this level of methemoglobin as these individuals generally have methemoglobin levels of 10-20% of total hemoglobin.

What do you mean by Methemoglobin?

Methemoglobin is a form of hemoglobin that has been oxidized, changing its heme iron configuration from the ferrous (Fe2+) to the ferric (Fe3+) state. Unlike normal hemoglobin, methemoglobin does not bind oxygen.

Methemoglobinemia can result from either congenital or acquired processes. Congenital forms of methemoglobinemia are due to autosomal recessive defects in the enzyme cytochrome b5 reductase.

Methemoglobin does not bind oxygen, thus effectively leading to a functional anemia. In addition, methemoglobin causes a leftward shift of the oxygen-hemoglobin dissociation curve.

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Final answer:

The consistency of methemoglobin levels in the Fugate family of Kentucky can be explained by genetic drift, a random process that can fix certain alleles in a small, isolated population.

Explanation:

The Fugate family of Kentucky, known for their blue skin due to methemoglobinemia, presents a case where genetic drift could explain the high consistency of methemoglobin levels.

Methemoglobinemia occurs when hemoglobin, which should normally be in the ferrous (Fe²⁺) form, is oxidized to the ferric (Fe³⁺) form, limiting its capacity to carry oxygen.

In the Fugates' isolated population, certain genetic variants, including those affecting methemoglobin levels, could have increased in frequency through genetic drift. This is a random process where allele frequencies within a small population change due to chance events.

Thus, a homogenous environment where the allele for methemoglobinemia is present and no significant selective advantage or disadvantage is attached to it could lead to stable levels of methemoglobin across the population due to genetic drift.

This process is distinct from balancing selection, such as the situation with the sickle cell trait where heterozygotes have a selective advantage in malaria-endemic regions, showing the complexity of genetic inheritance and its interaction with the environment.

Unlike sickle cell trait, no apparent selective advantage is associated with higher levels of methemoglobin, but the closed nature of the gene pool in the Fugate family likely led to the perpetuation and stability of these levels through genetic drift.

Which of the following summarizes the role of the hypothalamus?
A. It connects the endocrine and nervous systems and controls the pituitary gland.
B. It controls the rate at which cells burn fuels from food to produce energy.
C. It produces glucagon and insulin to control glucose levels in the blood.
D.It releases adrenaline as a response to fear, stress, and excitement.

Answers

I think the answer is A.

nomadic andsocieties living in marginal or ±uctuating environments

The hypothalamus is accountable for monitoring some of the metabolic procedures and other activities of the autonomic nervous system. It discharges and produces some of the neurohormones, known as releasing hormones or hypothalamic hormones, and these in return inhibit or stimulate the secretion of pituitary hormones.

Thus, the correct answer is option A, that is, hypothalamus connects the endocrine and nervous systems and controls the pituitary gland.

According to Darwin’s On the Origin of Species, what four factors affect evolution? Explain how each factor affects evolution.

Answers

The 4 factors affect evolution, according to Darwin’s Origin of Species are;

Variation

Heritability

Competition

Differential survival

Variation ensures that there is variability of population traits on which selection pressures will apply.

Heritability means that the traits must be able to be passed down to offsprings through gametes.

Competition occurs when the individuals in the population compete for limited resources

Differential survival occurs due to competition for resources and other selection pressures. This result to the survival of the strongest and weeding out of the weak from the population.






Describe how an organism has 2 sets of dna and the importance of homologous chromosomes

Answers

Most organisms are Diploid, and as a result, they will have received 2 copies of the chromosomes, or sets of chromosomes, from both their parents. This they will have 2 sets of Genes that will perform almost exact same process, except with different phenotypes.

These chromosomes need to be homologous, in order for new alleles and other genetic combinations to be made.

In fruit flies the allele for long wings (L) is dominant and the allele for short wings (l) is recessive. What is the likely genotype of a fruit fly with short wings?

A. Either LL or Ll
B. Either Ll or ll
C. Only LL
D. Only ll

Answers

It is D. Only ll
because if there was L in it at all it should have long wings.

In fruit flies the allele for long wings (L) is dominant and the allele for short wings (l) is recessive  the likely genotype of a fruit fly with short wings is D. Only ll.

What is the genotype of fruit flies?

In fruit flies, the dominant V allele produces lengthy wings, while the recessive v allele produces vestigial wings. Thus, flies with the genotype VV or Vv can have lengthy wings, and flies with the genotype vv can have vestigial wings.

There are varieties of traits, Dominant and recessive. For a dominant trait simplest single replica of gene is enough for a phenotype to appear. So LL and LI each will bring about lengthy wings. While in a recessive trait each copies have to be same for the phenotype to appear. simplest Il genotype will bring about brief wing fruit fly.

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What are the two correct terms for nitrate poisoning in newborn infants?

Answers

Final answer:

Nitrate poisoning in newborn infants is referred to as methemoglobinemia and blue baby syndrome; these terms describe the oxygen-deprivation symptoms that occur when infants consume water with high levels of nitrates.

Explanation:

The two correct terms for nitrate poisoning in newborn infants are methemoglobinemia and blue baby syndrome. When infants under the age of six months consume water with excess nitrates, they can develop methemoglobinemia, where hemoglobin is converted to methemoglobin, which cannot carry oxygen efficiently. This condition manifests as blue baby syndrome, characterized by symptoms such as shortness of breath and blue discoloration of the skin due to hypoxia. While the link between nitrates in drinking water and blue baby syndrome has been debated, with some suggesting other factors may contribute to the syndrome's outbreaks, elevated nitrate levels are still a recognized risk. The European Union's drinking water quality standards limit nitrate levels to less than 50 mg/L, while concerns about poisoning effects emphasize the importance of keeping hazardous substances away from children and following appropriate treatments for different types of poisoning.

Final answer:

The two terms describing nitrate poisoning in infants are methemoglobinemia and blue baby syndrome, conditions associated with high nitrate levels in drinking water and potential severe health risks.

Explanation:

The two correct terms for nitrate poisoning in newborn infants are methemoglobinemia, commonly known as blue baby syndrome.

This condition is characterized by a reduced ability of blood to carry oxygen, resulting in symptoms like shortness of breath and a blue tint to the skin.

Infants under six months are particularly at risk if they drink water containing nitrates above the Maximum Contaminant Level (MCL), which can lead to serious health issues and, if untreated, death.

While nitrate levels above 10 mg/L in drinking water are linked to methemoglobinemia, some studies suggest that other factors could also contribute to the syndrome. Nevertheless, maintaining safe drinking water standards is crucial for preventing such health risks in newborns.

Arrange the symbols to form a DNA molecule.

Answers

There are four different DNA nucleotides, each defined by a specific nitrogenous base: adenine (often abbreviated "A" in science writing), thymine (abbreviated "T"), guanine (abbreviated "G"), and cytosine (abbreviated "C")

You didn't include a picture so I don't know if this is exactly the answer.

A molecule that can be used as a molecular clock has a neutral mutation rate of one mutation per 20 million years. How many years ago did two species share a common ancestor if the molecules found in these two species differ by a total of six mutations?

Answers

Answer:

60 million years ago

Explanation:

The formula needed for this problem to calculate the correct number of years is:

Number of Base Pair Mutations × (Number of Years ÷ Mutation Rate) = Years of Evolution

Just like in math, simply substitute the corresponding numbers to get the desired answer:

6 Base Pair Mutations × (20,000,000 Years ÷ 1 Mutation) = 120,000,000 Years of Evolution

Then, divide the amount of time that separates these two species in half to determine how long ago their lineages shared a common ancestor. The formula needed for this part is:

Total Years of Evolution ÷ Number of Species (Lineages) = Years of Evolution From a Common Ancestor

Again, substitute the correct numbers in their place to get the answer:

120,000,000 Years of Evolution ÷ 2 Species/Lineages = 60,000,000 Years of Evolution From a Common Ancestor

Hope this helps :)

Final answer:

Two species that have molecules differing by six neutral mutations, given a mutation rate of one mutation per 20 million years, diverged from a common ancestor approximately 120 million years ago.

Explanation:

The question asks how long ago two species share a common ancestor if their molecules differ by six mutations, with the molecular clock indicating one mutation every 20 million years. To calculate the time of divergence, we multiply the number of mutations by the mutation rate. Since there are six mutations observed, and each mutation represents 20 million years of divergence, we perform the calculation of 6 mutations × 20 million years/mutation.

The calculation reveals that the two species diverged from a common ancestor 120 million years ago (6 × 20 million years = 120 million years). This period is based on the assumption that mutations as tracked by the molecular clock occur at a constant rate.

You have an simple of an unknown mineral. It can scratch fluorite but not quartz. What is it’s approximate hardness?

Answers

It's hardness on the mohs hardness scale is approximately 5.5

The approximate hardness of an unknown mineral ranges from 5-6 on the Mohs hardness scale.

How will you determine the hardness of any mineral or element?

The hardness of any mineral or element is estimated by the usage of the Mohs hardness scale or by scratching the unknown element with known substances.

The hardness of fluorite is four on the Mohs scale, while the hardness of quartz is seven which is harder as compared to fluorite.

If an unknown mineral makes a scratch on fluorite but not on the quartz, its hardness will definitely be higher than fluorite but lower than quartz.

Therefore, the approximate hardness of an unknown mineral ranges from 5-6 on the Mohs hardness scale.

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HELP 15 MINUET TIME LIMIT1!!!!!
Mutations are avoided during replication because DNA polymerase is able to Blank Space __________.
repair itself
repair the DNA
encode a message
add more codes

Answers

Mutations are avoided during replication because DNA polymerase is able to Blank Space repair the DNA.

DNA polymerase is an enzyme which carries out all forms of DNA replication. This enzyme catalyses the addition of nucleotide substrates to DNA in the 5' -3' direction during replication. Proof reading and error correction is done by the DNA polymerase. This enzyme can remove nucleotides from the end of the growing strand in order to correct mismatched bases.

Which of these BEST describes our current understanding about how species evolve over time?
A) As organisms use certain traits more frequently, each organism evolves over time.
B) Populations of organisms evolve rapidly due to genetic changes brought about by random mating patterns.
C) Traits that result in a greater number of mutations occur more frequently, causing the population to evolve.
D) Populations evolve as natural selection results in a change in the frequency of alleles within the population over time.

Answers

Populations evolve as natural selection results in a change in the frequency of alleles within the population over time.

The answer is D)- Populations evolve as natural selection results in a change in the frequency of alleles within the population over time.

When you climb a rope you change blank energy into blank energy

Answers

In every energy  transformation, some THERMAL ENERGY is released.

When you climb a rope, you change CHEMICAL ENERGY INTO KINETIC ENERGY.

 In the muscles cells in your body, CHEMICAL ENERGY is changed into KINETIC ENERGY.

Energy can neither be created nor destroyed, because of this, energy usually remain constant but it can be converted from one form to another.
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in the pyramid of energy model, what does the 10% rule mean

Answers

The 10% rule refers to the amount of energy available to the next trophic level.

Hope this helps!

-Payshence xoxo

the process of _____ results in the formation of sex cells

Answers

The answer is Meiosis
The process of Meiosis is required for sex cells.

FILL IN THE BLANK
A particular species of salamander was observed in California. Some groups of salamanders moved to the east, and a few others moved to the west. Over time, the two evolved and were no longer able to mate. This incompatibility was caused by (A. allopatric speciation, B. convergent evolution, C. coevolution) . The salamanders developed different reproductive behaviors, and the species were (A. mechanically isolate, B. behaviorally isolated, C. temporally isolated) .

Answers

Answer : The correct answer is -

1) A. allopatric speciation.

2)B. behaviorally isolated.

Allopatric speciation can be described as formation of new species when a biological population belonging to one species becomes geographically isolated and no gene flow occurs between the two during the course of evolution.

As per the given information in the question, a group of salamander population moved to east and another to west, which later evolved into two different population as they were unable to mate.

Thue, it represents allopatric speciation.

As the salamanders developed different reproductive behaviors, therefore it indicates behavior isolation, suggesting that they will perform different mating rituals.

Which organelle is responsible for breaking down the cells that once made up the tail of a tadpole

Answers

Hello,

Here is your answer:

The proper answer to this question is "lysosome".

 Your answer is lysosome.

If you need anymore help feel free to ask me!

Hope this helps!
lysosome

Your answer is lysosome

lysosomes are responsible for breaking down the cells that once made up the tail of a tadpole

Hope I helped! 

Let me know if you need anything else!

 ~ Zoe

what are human causes of sinkholes

Answers

Some are like when the construction workers drill and it causes vibrations.
Also Mining and lots of changes in weight.

Some causes are things in industrial work. For example building pipes underground and having it burst. Another example is in other places where water flows directly. Storm drains and pumping water out of the ground are some more examples.

what was the main environmental issue in this case?

A. Deforestation

B. Pollution

C. Overgrazing

D. Overpopulation

Answers

zit sure has to be B. Pollution

Answer:

Deforestation

Explanation:

The main environmental issue in this case is deforestation. Deforestation refers to the clearing, removal, or destruction of trees and vegetation from a forested area. This activity can have significant negative impacts on the environment.

What is a Nor'easter?

Answers

Im pretty sure A Nor’easter is a strong wind or storm blowing from the northeast. Hope this helps
A storm or wind blowing from the northeast, especially in New England

How do the two types of endoplasmic reticulum differ?
PLEASE HELP!

Answers

- RER = ● RER looks rough because it has particles called ribosomes attached to its surface
SER = ● SER looks smooth because it does not have ribosomes attached to its surface

- RER = ● the RER synthesises proteins
SER = ● the SER synthesises fats or lipids

- RER = ● the RER does not help in the detoxification
SER = ● the ser plays a crucial role in detoxifying many poisons and drugs




HOPE THIS HELPS. (please mark brainliest)

Answer:

the other person is right

Explanation:

what is the groove of an enzyme that holds the substrate

Answers

it's call the active site.

IN every energy transformation some blank is released

Answers

In every energy  transformation, some THERMAL ENERGY is released.

When you climb a rope, you change CHEMICAL ENERGY INTO KINETIC ENERGY.

 In the muscles cells in your body, CHEMICAL ENERGY is changed into KINETIC ENERGY.

Energy can neither be created nor destroyed, because of this, energy usually remain constant but it can be converted from one form to another.
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A newborn foal stands up to nurse from its mother. Is this an example of an innate or learned behavior?
A. innate
B. learned

Answers

Innate behavior as it is natural for it to nurse from its mother.
A. Innate

This is because the newboarn has to adapt

Both a grizzly bear and a slime mold are made of one or more cells that contain a nucleus and other organelles. What can you conclude they have in common, based on the concept of domains?


Answers.

hey are both in the domain Archaea.
They are both in the domain Bacteria.
They are both in the domain Eukarya.
They are both in the domain Animalia.

Answers

The best answer choice is:
                                            They are both in the domain Eukarya.

Explanation: Eukaryotes are organisms that are containing a nucleus. The nucleus is surrounded by a nuclear membrane, in which the chromosomes are located. Slime mold and grizzly bear are eukaryotic organisms that can live freely as single cells. Both have cells that contain a nucleus as well as other organelles bound within the nuclear membrane.

They are both in the domain Eukarya.

how is usable nitrogen obtained by anumals?

Answers

Animals obtain nitrogen by eating plants which has absorbed nitrogen from the soil.

Answer:

The animals get nitrogen from the plants. The nitrogen that is present in the environment is not used by the plants in the gaseous form. The bacteria that is found in the soil fixes nitrogen and makes it usable for the plants.

The plants uptake nitrpogen in the form of nitrates. The animals that feed on these plants uptake nitriogen from it.

The animals excrete the waste materials and release nitrogen in the environment.

Amoebic dysentery is caused by a parasitic amoeba. It reproduces in the _____.
spleen
liver
intestine
lungs

Answers

 Amoebic dysentery is caused by the amoeba Entamoeba histolytica. This protozoan exists in nature in the cyst form and is transmitted by contaminated food and water. In patients, the amoebas revert to trophozoites and invade the intestinal lining.

Your answer is C.

most water vapor in the atmospher is the source of
a. couds and rain.
b. pollution
c. carbon dioxide
d. wind.

Answers

Most water vapor in the atmosphere comes from greenhouse gases. 

Since carbon dioxide is a greenhouse gas, your answer is C. Carbon Dioxide 
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