Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. the use of mrna and reverse transcriptase is part of a strategy to solve the problem of _____. expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. the use of mrna and reverse transcriptase is part of a strategy to solve the problem of _____. electroporation nucleic acid hybridization post-translational processing post-transcriptional processing restriction fragment ligation request answer

Answers

Answer 1
The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of post-transcriptional processing. RNA is synthesized in the process of transcription and then transferred to the cell cytoplasm where it is translated into protein. Post transcriptional modification or processing is a process where the primary transcript RNA is converted into mature RNA. 
Answer 2
Final answer:

The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of post-transcriptional processing when expressing a cloned eukaryotic gene in a bacterial cell.

Explanation:

The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of post-transcriptional processing when expressing a cloned eukaryotic gene in a bacterial cell.

Eukaryotic mRNAs must undergo several processing steps before they can be translated into a protein. The additional steps involved in eukaryotic mRNA maturation create a molecule that is much more stable than a prokaryotic mRNA.

By using mRNA as a template, reverse transcriptase can make a complementary DNA (cDNA) copy of the mRNA, which can then be used to produce a cDNA library of only the expressed genes in the cell, excluding introns and other non-coding sequences.

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Related Questions

The spinal cord is protected by the

Brain Stem
Lower Cord
Back Bone
Tail Bone

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the back bone is the correct answer

Modern h. sapiens differ from the archaic form in that moderns have

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Modern H. sapiens differ from the archaic form in that moderns have a high, vertical forehead, a mental eminene; and lack occipital bun. Modern homo sapiens have distinguishing features such as they have a short base, a high braincase, back of the skull is rounded and indicates a reduction in the neck muscles, face is reasonably small with a projecting nose bone among other features that makes them different from the other sapiens. 

Final answer:

Modern Homo sapiens are distinguished from archaic forms by their larger, more complex brains, smaller faces, jaws, and front teeth, and changes in skull morphology such as less pronounced brow ridges and the development of a prominent chin. They also have a more elaborate tool kit, including the blade tool industry.

Explanation:

Differences between Modern Homo sapiens and Archaic Homo sapiens:

The evolutionary journey from archaic Homo species to modern Homo sapiens has been marked by significant changes in physical anatomy and behavior. Modern humans are biologically distinct from their archaic predecessors in a number of ways. One of the most notable physical differences is the size and structure of the brain. Modern H. sapiens have a much larger brain capacity, averaging around 1,200 to 1,400 cubic centimeters, just like some archaic forms, but with a more developed prefrontal cortex and smaller face, jaws, and front teeth. Another distinctive characteristic of modern humans is their skull morphology, which generally includes a rounder skull, less prominent brow ridges, and a prominent chin, unlike their archaic counterparts who had thicker skulls and more pronounced brow ridges.

Furthermore, the behavioral changes that accompany these physical evolutions are substantial. Modern humans demonstrate a significant leap in tool-making abilities, transitioning from the Mousterian prepared-core flake tools to a more sophisticated blade tool industry. This advancement allowed the crafting of tools from a wider variety of materials including bone, antler, and ivory, which facilitated the development of a rich material culture in the Upper Paleolithic period. Additionally, modern H. sapiens displayed advanced cognitive functions and social behaviors, leading some researchers to speculate about their use of language and complex social structures.

Which of the following affects the speed of a planet's revolution?

Shape of the planet
Gravity of the sun
Temperature of the sun
Atmosphere of the planet

Answers

i would say B) because the rest dosnt quite make sense to me and A) the  gravity of the sun would still be the same despite the shape if we have an triangle block and  an sphere then drop the they are going to drop at the same speed the gravity is not going to sphere is not going to fall faster 

The gravity of the sun affects the speed of a planet's revolution.

What is revolution?

Revolution refers to the orbital movement of a celestial body, such as a planet, around another object, typically a star. It is the path or trajectory followed by the celestial body as it completes a full orbit around its central object.

During revolution, the celestial body maintains a relatively constant distance and orientation with respect to the central object due to the gravitational attraction between them. This orbital motion defines the planet's year or orbital period, which is the time it takes for the planet to complete one revolution. The revolution of a planet is a fundamental aspect of its motion within a solar system or galaxy.

The speed at which a planet revolves around its star, such as the sun, is primarily determined by the gravitational pull between the planet and its star. The strength of the gravitational force depends on the mass of the star and the distance between the planet and the star. According to Kepler's laws of planetary motion, planets that are closer to their star experience a stronger gravitational force, leading to higher orbital speeds.

Conversely, planets that are farther away experience a weaker gravitational force, resulting in slower orbital speeds. Therefore, the gravity of the sun directly influences the speed of a planet's revolution around it.

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__________ are underground areas that have the ability to contain and provide water sources.a.shamalsb.wadisc.seasd.aquifers please select the best answer from the choices provided.

Answers

Hello User

Answer: aquifers are underground areas that have the ability to contain and provide water 

Note: An aquifer is a group of rocks that water is able to flow through.

I hope this helped
-Chris

Answer:

Aquifers

Explanation:

Groundwater is an important source of water. It is the water present in the deeper layers of soil below the water table. Some of the important sources of groundwater are rainfall, rivers, ponds, etc. When it pours, water goes down in the soil layers to fill the spaces present below the ground. When the groundwater is accumulated between the hard rock layer, it becomes aquifer. The aquifers serve as a source of fresh water as the water present in them can be pumped out using techniques such as tube wells.

Mitochondria transfer ________ energy from ________ to atp; chloroplasts transform ________ energy into the chemical energy of atp. food . . . light . . . nuclear chemical . . . food . . . light light . . . food . . . kinetic food . . . light . . . chemical

Answers

Final answer:

Mitochondria transfer chemical energy from food to ATP, acting as the cell's energy producers. Chloroplasts transform light energy into chemical energy through the process of photosynthesis.

Explanation:

The mitochondria transfer chemical energy from food to ATP (Adenosine Triphosphate). ATP is the main energy source for the majority of cellular functions. However, it is not a long-term energy storage molecule. That's where the food we consume comes into play. When we consume food, our bodies break down the organic molecules within, releasing energy that is then converted into ATP.

Chloroplasts, on the other hand, are responsible for taking in energy from light and converting it into chemical energy via the process of photosynthesis. In photosynthesis, light energy is absorbed by chlorophyll, which drives the synthesis of glucose and other nutrients. These glucose molecules can then be used by the mitochondria to produce ATP.

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Mitochondria convert chemical energy from food into ATP, while chloroplasts convert light energy into ATP's chemical energy.

Mitochondria are responsible for the process of cellular respiration, where they use the chemical energy stored in food molecules to produce ATP, which is the energy currency of the cell.

Chloroplasts, found in plant cells, convert light energy into chemical energy during the process of photosynthesis, particularly in the light-dependent reactions where ATP is produced alongside NADPH

The phase of cell division during which the chromatids are pulled apart and move to the opposite

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The phase of cell division during which the chromatids are pulled apart and move to the opposite is the anaphase.

What is the ratio of heterozygous offspring to total offspring in the Punnett square?

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I think the the ratio of heterozygous offspring to total off spring in the Punnet square is 4/4. 
A punnet square is a tool that can be used to predict the genotypes of potential offspring in a given cross, like the one above. It can be used to find the probability that a certain allele combination will occur in offspring. 

In a Punnett square representing a cross between two heterozygous parents for one gene, the ratio of heterozygous offspring to total offspring is 1:2, or alternatively 50%. In other words, for every four offspring produced in such a cross, two are expected to be heterozygous.

When two heterozygous parents are crossed (Bb x Bb), the possible genotypes of the offspring are BB, Bb, bB, and bb. Here, we notice that Bb and bB are actually the same genotype as the letters represent alleles from different parents but do not designate the order of alleles on the chromosome. Therefore, if we consider Bb and bB as identical, and sum the heterozygous genotypes (Bb + bB), we get two out of four possible offspring that are heterozygous. Since a Punnett square has four boxes, and each box represents an equally likely outcome, the ratio is 2 heterozygotes out of 4 total offspring, which simplifies to a 1:2 ratio (50% of the offspring). This is consistent with the Mendelian inheritance predictions for a monohybrid cross.

How long does a letter take from boston to amsterdam?

Answers

Anywhere from 8-14 days.

A 3-base deletion in the aauaaa sequence in the 3' untranslated region of an mrna that eliminates the aau, thereby preventing rna polymerase from polyadenylation the mrna would have the effect of:

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A 3-base deletion in the AAUAAA sequence in the 3' untranslated region of an mRNA that eliminates the AAU, thereby preventing RNA polymerase from polyadenylation the mRNA would have the effect of; decreasing the number copies of the polyeptide that would be translated from this mRNA. A deletion is a mutation in which a part of a chromosome or a sequence of DNA is lost during DNA replication. 

In _____ the heterozygote's phenotype is somewhat intermediate between the two homozygotes. select one:
a. codominance
b. complete dominance
c. incomplete dominance
d. epistasis

Answers

In incomplete dominance the heterozygote's phenotype is somewhat intermediate between the two homozygotes.

An example of this is when you cross a red homozygote flower with a white homozygote flower and you get a pink phenotype.

Ive attached a pic that might help

I hope this helps! I'm happy to answer any other question you might have :)

When alzheimer disease appears in middle age rather than old age, it:
a. progresses more slowly.
b. progresses more rapidly.
c. causes death within a year.
d. is benign?

Answers

When alzheimer disease appears in middle age rather than old age; it progresses more rapidly. Alzheimer's disease that results from the brain cell death. It is a neurodegenerative disease; such that there is progressive brain cell death that happens over a period of time. The brain tissue gets fewer and fewer nerve cells and connections. 

Most of the energy in the typical animal cell is produced in the __________.

Answers

Rough and smooth endoplasmic reticulum

The main action of the fibularis longus is to __________. invert the foot dorsiflexes the foot evert the foot plantar flexes the foot

Answers

The main action of the fibularis longus is to evert the foot. Fibularis longus is a superficial muscle in the lateral compartment of the leg, and its main function is to evert and plantarflex the ankle. The muscle originates at the head and the upper body of the fibula and the intermuscular septa. It inserts at the plantar side of the medical cuneiform and first metatarsal bone.

the answer is 10 feet in total

Explanation:

T cells produce and secrete factors that do not directly interact with invading microorganisms but that augment the body's defense mechanisms. these molecules are

Answers

cytokines since they signal cells around them

​the condition known as _____ is characterized by hypertension, edema, and proteinuria.
a. ​preeclampsia
b. ​uterine prolapse
c. ​oophorectomy
d. ​eclampsia

Answers

 A. Pre-eclampsia

Pre-eclampsia also known as pregnancy induced hypersion or toxemia. It is a pathological condition in pregnancy that is characterized by hypertension, edema and proteinuria. However, pre-eclampsia is a symptom that is associated with late pregnancy that encompasses both convulsive and non-convulsive stage.

Your kidneys have an important effect on blood pressure. The kidneys controls plasma volume by controlling how much _____________________.

Answers

The kidneys control plasma volume by controlling how much Na and water are reabsorbed.  

The juxtaglomerular apparatus is a structure formed by the distal convoluted tubule and the glomerular afferent arteriole which regulate blood pressure and filtration. The components of the apparatus are macula densa which detects Na concentration and juxtaglomerular cells which sense the blood pressure. When blood pressure and Na decrease, the juxtaglomerular cells then release an enzyme called renin. Renin converts angiotensinogen into angiotensin I, then angiotensin I is converted to angiotensin II which causes blood vessels to contract (elevate the blood pressure). Angiotensin II also stimulates the adrenal gland to secrete aldosterone which stimulates more Na and water reabsorption (also elevates the blood pressure).

Answer:

C. Water a person excretes

Explanation:

The kidneys controls the amount of water a person excretes which effects the blood pressure by controlling the volume of plasma.

competitive exclusion principle definition biology

Answers

competitive exclusion principle. In ecology the competitive exclusion principle, sometimes referred to as Gause's Law is a proposition which states that two species competing for the same resources cannot coexist if other ecological factors are constant.

In which stage of the Calvin cycle does the plant cell produce energy for storage?

Answers

For anyone who believes that the answer is "regeneration," that's incorrect.

natural killer cells definition

Answers

Natural killer cells (NK cells) are an aggressive part of the immune system that plays an important role in eliminating cancer cells and viral-infected cells from the body.
Final answer:

Natural killer (NK) cells are cytotoxic lymphocytes that play a vital role in innate immunity by identifying and destroying cells that appear abnormal or are infected, primarily through changes in major histocompatibility complex (MHC) I expression. They induce apoptosis in these cells without the need for prior antigen sensitization, making them a swift response against infections and cancers.

Explanation:

Natural killer (NK) cells are a critical component of the innate immune response, functioning as cytotoxic lymphocytes. These NK cells patrol the body, seeking out cells that exhibit 'non-self' proteins on their membranes or that have foreign or abnormal markers indicative of infection or cellular transformation, such as that which occurs in cancer. Unlike T cells of the adaptive immune response, NK cells do not require prior sensitization to specific antigens and thus provide a rapid response to invasions or cellular abnormalities.

One way NK cells are able to discern unhealthy cells from healthy ones involves the detection of changes in the expression of major histocompatibility complex (MHC) I molecules on the surface of nucleated cells. Healthy cells present normal 'self' peptides via MHC I to the immune system, signaling their healthy state. Conversely, cells that are infected or becoming cancerous often have altered MHC I expression, which is recognized by NK cells, triggering a targeted response.

Once an NK cell identifies a potential target, they can induce apoptosis, or programmed cell death, within the aberrant cell. Through the release of cytotoxic granules, NK cells deliver potent molecules like perforin and granzymes, which penetrate the target cell's membrane and initiate the apoptosis cascade, leading to cell death and the removal of the threat they posed.

Which of these people has the lowest risk of suffering serious consequences from a foodborne illness?

Answers

For the answer to the question above, I think that lowest risk of suffering serious consequences from a foodborne illness is "that the person who is recovering from a surgery".
I hope my answer helped you. Have a nice day!

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EXTRA POINTS
The overuse of antibiotics is causing a rise in the number of bacteria which are resistant to antibiotics. Which mechanism would most likely account for this?

Select one:
a. Through competition for reduced resources, the antibiotic-resistant bacteria thrive.
b. Through mutation, the bacteria change into other species.
c. Through natural selection, a few surviving antibiotic-resistant bacteria multiply.
d. Through movement to other habitats, bacteria find more places to live.

Answers

the answer is C your welcome!

Which cells are responsible for his ability to see the objects and people?

Answers

these cells are called rods

Which of the below is an example of an indirect food additive?

Answers

in this question you have written which of the below is an example of indirect food additive now where is the below option

A substitute is a good that is​ _____ another​ good, and a complement is a good that is​ _____ another good.
a. produced in place​ of; sold with
b. consumed in place​ of; produced with
c. consumed in place​ of; consumed together with
d. consumed together​ with; consumed in place of

Answers

The right option is c. consumed in place of; consumed together with

A substitute is a good that is consumed in place of another good, and a complement is a good that is consumed together with another good. Substitutes serve in place of another and complements make something seem better.






Brad seems to be in a continuous state of anxiety though he is unable to identify the source of his feelings. the most likely diagnosis for brad is:

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The answer is generalized anxiety. The symptoms of this disorder is that the person tend to always expect that there is a disaster that will going to happen and also they can’t stop worrying about health, money, work, family and everything under the sun. Generalized anxiety disorder is defined by exaggerated anxiety, excessive worrying about everyday life events with no basis and obvious reasons.

interstitial cells surrounding seminiferous tubules produce testosterone in response to __________.

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interstitial cells surrounding seminiferous tubules produce testosterone in response

lh

Which molecule contributes to the greenhouse effect?

Answers

Methane Gas.     It would NOT be Nitrogen, Helium, or Oxygen gasses.                                                

The molecule that contributes to the greenhouse effect are water molecules and methane.

What is the greenhouse effect?

The greenhouse impact is the manner wherein warmth is trapped near Earth's floor by “greenhouse gases.” These warmth-trapping gases may be idea of as a blanket wrapped round Earth, retaining the planet toastier than it'd be with out them.

Carbon dioxide: Made of 1 carbon atom and  oxygen atoms, carbon dioxide molecules make up a small fraction of the atmosphere, however have a big impact on climate.The molecules that make a contribution to the greenhouse impact are people with a nontrivial vapor stress at Earth’s floor temperature (in any other case they won’t be capable of evaporate into the atmosphere) and that are compounds withinside the experience of together with  or extra elements.

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The natural and synthetic drugs derived from morhphine are know as

Answers

Heroin, Oxycodone, Oxymorphone, Hydromorphone.

These are some.

Which structures are common to both prokaryotic and eukaryotic cells? check all that apply. vacuole cell membrane golgi body ribosome cytoplasm endoplasmic reticulum?

Answers

The structures that are common to both prokaryotic and eukaryotic cells are plasma membrane, ribosomes, cytoplasm, and DNA
All cells have a plasma membrane, ribosomes, cytoplasm, and DNA. However, Prokaryotic cells lack a nucleus and membrane bound structures while the eukaryotic cells have a nucleus and membrane bound structures called organelles. 

The structures that are common to both prokaryotic and eukaryotic cells are:

Cell membraneRibosomeCytoplasm

The cells of multicellular organisms are different in their function, because they are structurally different, but they all agree with a common pattern.

Prokaryotic cells, like eukaryotic cells, have a plasma membrane, but lack internal membranes, which form organelles.

Main common characteristics between eukaryotic and prokaryotic cells:

In both cell types, DNA is the genetic material.Both cell types have plasma membranes as cell boundaryThey have ribosomes for protein synthesis.The cytoplasm is common to both eukaryotic and prokaryotic cells, although they contain different types of organelles.

Therefore, we can conclude that the structures that are common to both prokaryotic and eukaryotic cells are: cell membrane, ribosomes, cytoplasm.

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Although the open ocean is among the least productive ecosystems, it contributes greatly to the overall productivity of the biosphere. How can this situation be explained?

Answers

Final answer:

The open ocean contributes greatly to the biosphere's productivity due to the massive scale of ocean ecosystems and the vital role of phytoplankton in oxygen production through photosynthesis. The ocean upwelling also redistributes nutrients, enhancing productivity.

Explanation:

Although the open ocean is among the least productive ecosystems, it plays a significant role in global productivity, largely due to the extent of ocean ecosystems which encompass over 70% of the Earth's surface. The ocean's vastness houses numerous species, ranging from large marine mammals to the tiniest of organisms, such as phytoplankton.

Phytoplankton are crucial for the biosphere as they release over 50% of the oxygen produced on Earth through photosynthesis. This process not only contributes to the oxygen we breathe but also forms the foundation of the marine food web. The neritic zone, with its high biodiversity and productivity, plays a significant role in supporting marine life and fisheries. However, it's the sheer scale of the open ocean that allows its contribution to overall biosphere productivity.

Moreover, phenomena such as ocean upwelling help redistribute nutrients from the ocean floor back to surface waters, aiding in the productivity of these ecosystems. Therefore, the open ocean's significant contribution to Earth's productivity can be explained by the large area it covers, the oxygen production by phytoplankton, and the mechanisms that redistribute nutrients within these aquatic systems.

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The area of a rectangular garden is given by the trinomial x2 + x 42. What are the possible dimensions of the rectangle? Use factoring. a rectangular box has a volume of 105 cubic centimeter the width of the rectangular box is X centimeters the length is 2x - 3 cm and the height is 3 cm What does Nixon say has changed now that America is the strongest and richest nation in the world I hail from Crete's broad land, I'm proud to say,and I am a rich man's son. And many other sonshe brought up in this palace, born in wedlock,sprung of his lawful wife. Unlike my mother.She was a slave, a concubine he'd purchased, yes,but he treated me on par with all his true-born sons Castor, Hylax's sonThis is an example of which epic convention?a. lengthy speechb. digressionc. epic similed. divine interventionI know it is either a or d.... HELP!!! The federal laws of the united states were mainly derived from the common law system of england. a. True b. False Which function represents a reflection of f(x) = 5(0.8)x across the x-axis? A. g(x) = 5(0.8)x B. g(x) = 5(0.8)x C. g(x) = 1/5(0.8)x D. g(x) = 5(0.8)x at the market,8 apples cost $4.How much do 9 apples cost? Coleridge uses his speaker's point of view to describeA. the possible thoughts and feelings of creatures and plants in natureB. one person's feelings about nature and its many characteristicsC. one person's thoughts on the lesson nature provides without work How do institutions like religion and politics play a role in creating or maintaining beliefs and preconceived notions Write a program that asks the user for the name of a text file. the program should display the last 10 lines of the file on the screen In "And OhThat the Man I Am Might Cease to Be" by D. H. Lawrence, what is the emotional state of the speaker? Support your answer with details from the poem. When muslims bow toward mecca, the holy city of islam, five times a day at fixed times to pray to god, and christians participate in the celebration of communion to commemorate the life, death, and resurrection of jesus, they are engaging in:? Which post war events contributed the most to american distrust of the soviet union? Urban legends are our modern-day folklore. Describe a cautionary urban legend that you have heard and describe what it was supposed to caution you against. If you were faced with a similar situation, would the urban legend influence your decision? Use the Factor Theorem to determine whether the first polynomial is a factor of the second polynomial. x - 2; 4x2 - 3x + 22 What is lanugo? very fine, soft hair on the face and arms of people with anorexia nervosa a sudden drop in blood pressure from excessive dieting a side effect of liposuction surgery a supplement claimed to increase metabolism 1 points? which of the following best describes why malory's morte d'arthur is considered a medieval romance What did Spain receive in the 1763 Treaty of Paris in return for its territory east of the Mississippi River?A. Havana, Cuba B. Pensacola, Florida C. St Augustine, Florida D. New Orleans, Louisiana This would help a lot Please and thank you :) Do popular cartoon characters on food packages influence children's food choices Why is the concept of a stigma so critical to the concept of deviance?