In the process of genetic engineering, what is the correct order of the steps?

Answers

Answer 1
Final answer:

The correct order of steps in the process of genetic engineering is: identify desirable gene, isolate gene, insert gene into vector, introduce vector into host organism, allow replication and expression, and screen for successful incorporation and expression.

Explanation:

The correct order of steps in the process of genetic engineering is as follows:

Identify the desirable trait or gene that you want to introduce into the organism.Isolate the gene from the source organism using molecular cloning techniques.Insert the gene into a vector, such as a plasmid.Introduce the vector into the host organism, often using a technique called transformation.Allow the host organism to replicate and express the foreign gene.Screen for successful incorporation of the foreign gene and verify its expression.

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Related Questions

The most important of the secondary lymphoid organs in the body are the lymph nodes.
a. True
b. False

Answers

The answer is a. True

The long band of nerve fibers that connects the brain's hemispheres is:

Answers

 The answer is Corpus callosum. The largest structure of the human brain, consisting of the two cerebral hemispheres connected by the corpus callosum and covered by the cerebral cortex. Corpus callosum is a long, thick band of nerve fibers that connects the left and right hemispheres of the brain and allows communication between them. Development of the CC makes communication between the hemispheres more efficient.

Proteins that are targeted to different cellular compartments other than the cytosol are made on the ribosomes attached to the endoplasmic reticulum. in order for ribosomes to attach to the endoplasmic reticulum, which proteins that are attached to the ribosome interact with proteins associated with the endoplasmic reticulum

Answers

Signal recognition particle


Explain how the cell cycle is normally controlled, including reference to the role of tumor-suppressor genes:

Answers

The cell cycle is composed of S (synthetic) phase and M (Mitotic) phase. In between are G1 and G2 phases. cyclin-dependent kinases propel cell division. Their activation results in the progression of a cell from one phase to another while their inactivation arrests a cell in a phase. In case of damage to DNA during a cell cycle, transcription factors p53 (a renowned tumor suppressor) are recruited. P53 inhibits cyclins-dependent kinases and also induces cell apoptosis if DNA is not repaired.






Final answer:

The cell cycle is controlled by the balance between proto-oncogenes and tumor suppressor genes. Mutations in these genes can result in uncontrolled cell growth, possibly leading to cancer. Tumor suppressor genes normally prevent division of cells with damaged DNA, but when mutated, they may fail, contributing to cancer development.

Explanation:

Control of the Cell Cycle and Tumor Suppressor Genes.The cell cycle is tightly controlled by a balance between proto-oncogenes and tumor suppressor genes to ensure that only healthy cells replicate. Tumor suppressor genes act as the 'brakes' of the cell cycle, preventing cells with damaged DNA from dividing. When these genes are mutated, they fail to function properly, resulting in uncontrolled cell division, which can lead to cancer.

Proto-oncogenes, on the other hand, when mutated, turn into oncogenes that promote cell division even if DNA is damaged. Mutations in these genes often occur due to environmental factors that damage DNA or through inherited genetic predispositions. The combined effect can lead to the development of cancer through uncontrolled cell growth.

Key proteins coded by tumor suppressor genes, such as Rb, p53, and p21, put up roadblocks to cell cycle progression until certain conditions are met. If these proteins are missing or dysfunctional due to mutations, it can result in the loss of cell cycle control and the onset of cancerous growths.

Why does the h+ gradient across the membrane dissipate when the light goes off? what happens to the rate of atp production?

Answers

The reaction is driven by the absorption of light energy. So the H+ gradient across the membranes dissipates when the light goes off and the reaction is stopped.

What are phototrophs?

The organisms that capture the particles of light and convert them into chemical energy are called phototrophs. Organisms capture this light with specialized pigments. The light is captured in the form of electromagnetic energy.

An enzyme called the H+ driven ATP synthase moves the H+ ions through the ATP synthase. The ATP synthesis continues until the H+ gradient runs out of energy to drive the reaction forward.

Thus the reaction continues for as long as the light is being absorbed. The H+ gradient that is generated is persisted even after the light goes off. The gradient is persisted till the H+ ions move through the ATP synthase.

Thus, the cell used light for the generation of ATP and it is then stored for use later. ATP is a chemical battery and H+ is an electrochemical battery.

Therefore, the reaction is driven by the absorption of light energy. So the H+ gradient across the membranes dissipates when the light goes off and the reaction is stopped.

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Extrusive igneous rock _____. forms beneath the earth's crust usually cools very slowlyb forms on the earth's crust has large mineral crystals

Answers

Extrusive igneous rock forms on the earth's crust has large mineral crystals.

Answer:

--forms on the earth's crust--

Explanation:

It can't be cools slowly because in the reading (on my assignment) it kept on stating that the extrusive igneous rock cool very quickly, so it cant be cools slowly...

Next we have Forms beneath the earths crust, I don't have much info on this one but I know for a fact that the extrusive igneous rock forms as soon as the volcano explodes its lava out then it quickly cool, So I'm guessing that its not this one either.

Next we have- forms on the earth's crust, Since a volcano is on the crust of the earth that means that the rock would be too! Since the rock can only be formed (I don't think that is a word lol) right as soon as the magma becomes lava then the rock cools and then becomes an extrusive igneous rock-

Last we have - Has large crystals, This is not possible since the rock cools extremely fast it does not have the time for bigger crystals to form, if that makes sense lol. therefore this cannot be the answer..

After all this we finally have our answer!! The answer is --Forms on earths crust--   I HOPE THIS HELPED YOU!! GOOD LUCK!! and yes this question was posted 2 years ago- lol

Explanation:

Which two types of tissue have the greatest ability to repair themselves?

Answers

Epithelial  and  connective  tissues  have  the   greatest  ability  to  repair  themselves. Epithelial  and   connective  tissues  replace  damaged  or  dead  cells   from  a  supply   of adult  stem  cells.  This tissue  contains  pools  of   stem  cells  which  have  enormous  proliferative  and  self-renewing  ability  and   which   give  rise  to  more  than  one  type  of  cell.

What chemicals regulate the cell cycle how do they work?

Answers

Final answer:

The cell cycle is regulated by chemicals, primarily proteins called cyclins and Cyclin-dependent kinases (CDKs). Cyclins fluctuate in concentration throughout the cycle, activating CDKs that stimulate the cell to progress stages by phosphorylating target proteins. This regulates the timing and progression of the cell cycle.

Explanation:

The cell cycle is a complex process regulated by a series of chemicals and proteins. The two main chemicals are proteins known as cyclins and cyclin-dependent kinases (CDKs). Cyclins increase and decrease in concentration throughout different parts of the cell cycle, binding to CDKs and activating them. These activated CDKs then stimulate the cell to progress to the next phase by phosphorylating target proteins. When there are high levels of cyclins, the cell moves into the mitosis phase. After mitosis, the cyclin levels decrease, causing the cell to exit the cell cycle and enter the G0 phase. Therefore, these two chemicals work together to control the progression and timing of events in the cell cycle.

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Final answer:

The cell cycle is regulated by chemicals namely cyclins, cyclin-dependent kinases (Cdks), and regulatory molecules. These chemicals can either promote or halt the cycle depending on cell conditions. Any dysfunction in these chemicals can cause unchecked cell division, eventually leading to cancer.

Explanation:

The cell cycle is regulated by various types of chemicals which include cyclins and cyclin-dependent kinases (Cdks), along with regulatory molecules that either promote or hinder the progress of the cycle. The cell moves through the cycle under the supervision of these control molecules, which determine whether conditions are correct for the cell to transition to the next stage. Cyclins and Cdks regulate transitions through checkpoints in the cell cycle, while negative regulator molecules, such as tumor suppressor proteins, can halt the cycle if specific requirements are not met.

On the other hand, Cdk inhibitors are molecules that block the full activation of Cdks to arrest the cell cycle until an event they are monitoring is completed. Any changes in these regulatory molecules can result in unchecked cell division leading to cancer. Therefore, these chemicals maintain the balance between cell division and cell death and help in the proper functioning of cell cycle.

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An arithmetic progression involves the addition of the same quantity to each number. Which might represent the arithmetic growth of agricultural production? 1, 4, 6 1, 11, 111 1, 5, 10 2, 4, 6

Answers

An arithmetic progression involves the addition of the same quantity to each number. 2,4,6 represents the arithmetic growth of agricultural production.

Answer: 2, 4, 6

Explanation:

Given statement: An arithmetic progression involves the addition of the same quantity to each number.

1, 4, 6

Here, [tex]4-1=3\\6-4=2[/tex]

Thus, It does not involve the addition of the same quantity to each number.

Hence, It does not represent the arithmetic growth of agricultural production.

1, 11, 111

Here, [tex]111-11=110\\11-1=10[/tex]

Thus, It does not involve the addition of the same quantity to each number.

Hence, It does not represent the arithmetic growth of agricultural production.

1, 5, 10

Here, [tex]5-1=4\\10-5=5[/tex]

Thus, It does not involve the addition of the same quantity to each number.

Hence, It does not represent the arithmetic growth of agricultural production.

2, 4, 6

Here, [tex]4-2=2\\6-4=2[/tex]

Thus, It involves the addition of the same quantity i.e. 2 to each number.

Hence, It might represents the arithmetic growth of agricultural production.

A patient with chronic cholecystitis reports pruritus, clay-colored stools, and voiding dark, frothy urine. which laboratory analysis is a priority in the nurse's assessment of this patient? lipase level total bilirubin liver function tests white blood cell count

Answers

The answer would be: total bilirubin

Bilirubin is the waste that needs to be dumped by the gallbladder. In chronic cholecystitis, the common bile duct might be obstructed. This leads to increased level of bilirubin, mostly conjugated bilirubin. Pruritus, clay-colored stools, and voiding dark, frothy urine could be caused by hyperbilirubinemia(increased level of bilirubin in the blood). 

Which of the following cell organelles contains instructions for making proteins?

Answers

Answer:

ribosomes

Explanation:

As mentioned earlier, ribosomes are the molecular machines responsible for protein synthesis. ...

In eukaryotes, ribosomes get their orders to synthesize core proteins, where segments of the DNA (genes) are transcribed to produce messenger RNA (mRNA).

Answer:

Ribosome

Explanation:

Because they make the protein that's needed for the cell

The nurse has administered intravenous (iv) nitroglycerin (nitro-bid) to a patient. the nurse later discovers that the patient's systolic blood pressure is 65 mm hg. what should the immediate nursing intervention be in this situation?

Answers

The immediate nursing intervention should be Discontinuing the nitroglycerin (Nitro-Bid) infusion. Nitroglycerin is a group of drugs called nitrates. NItroglycerin dilates blood vessels making it easier for blood to flow through them and easier for the heart to pump. Nitro-Bid is used to prevent attacks of chest pain.The should therefore discontinue the administration of the drug since the systolic pressure lower than normal.

Refers to the area(s) in in the midportion of the shafts of long bones where bone cells are formed so that the cartilage-model bones of the fetal skeleton begin ossifying, from the center outward, to form bone shafts.

Answers

The primary ossification center is the area in the midportion of long bone shafts where ossification begins during fetal development, forming the bone shafts. Secondary ossification centers form after birth at the ends of the bones, and these centers, together with the epiphyseal plate, allow bone lengthening until maturity.

The area in the midportion of the shafts of long bones where bone cells are formed for the ossification of cartilage-model bones of the fetal skeleton is referred to as the primary ossification center. This center initiates ossification during fetal development, converting the cartilage to bone tissue and forming the bone shafts. The process continues with the formation of secondary ossification centers after birth, which contribute to the formation of the ends of the bones.

Endochondral ossification is the specific process where the hyaline cartilage model is turned into bone, typically beginning by the twelfth fetal week. During early development, the skeleton is mostly made of cartilage. This cartilage then undergoes ossification, beginning at a primary ossification center in the diaphysis, or middle of the bone, and later at secondary ossification centers in the epiphyses, or bone ends.

The remaining cartilage at the end of long bones and the epiphyseal plate or growth region allows for continued bone lengthening until skeletal maturity is reached when the epiphyseal plate eventually disappears and the bone regions fuse.

A nurse is assessing a primigravida who was admitted in early labor after her membranes have ruptured. she is at 41 weeks' gestation. her contractions are irregular, and her cervix is dilated 3 cm. the fetal head is at station 0, and the fetal heart rate tracing is reactive. how can the nurse help the client facilitate labor

Answers

The answer would be: take a walk around the unit with her. 

The patient is not ready for labour because of her uterus has an irregular contraction and the cervix is only 3cm. Taking walk around can increase the frequency and the intensity of uterus contraction. The patient should be accompanied to ensure her safety.

11. Which of the following is not true?
A) ATP is formed during both photosynthesis and cellular respiration
B) Both plant cells and animal cells carry out cellular respiration
C) Carbon dioxide is released during photosynthesis and used during cellular respiration
D) Oxygen is used during cellular respiration and released during photosynthesis
13. Glycolysis requires
A) ATP
B) Oxygen
C) Sunlight
D) NADP+,

Answers

11. C) Carbon dioxide is released during photosynthesis and used during cellular respiration. During photosynthesis Carbondioxide is fixed to carbohydrate and oxygen is released. and hence there is no evolution of carbon dioxide. In cellular respiration no carbodioxide is used instead it is released.
13. A) ATP. It is used in various steps during glycolysis

Alcoholic beverages
a.dilate the blood vessels and
b.increase the amount of water removed from the blood. how would factors a and b affect blood pressure?

Answers

Blood vessels dilation causes the resistance inside the vessels to decrease, and thus decreases BP.

Water removed from the blood causes blood volum to decrease, and thus decreases BP.

In comparing the five-kingdom system of classification with the six-kingdom system, all ___________ are grouped in one kingdom in the five-kingdom system, but are divided into two kingdoms in the six-kingdom version.
A) animals
B) bacteria
C) plants
D) protists

Answers

B) Bacteria is the answer!!

Bacteria is the answer to this question

A decrease in the oxygen-carrying ability of the blood, for any reason, is a condition known as ________.

Answers

ANEMIA

Anemia is a condition of the blood where there is not enough red blood cells or hemoglobin. Hemoglobin has an iron core and is the main part of the red blood cells where oxygen from the lungs binds. IF hemoglobin is too low or abnormal delivery of oxygen to the body will be altered. There are various reasons for anemia to happen. This cane be due to abnormal cell development, deficiency in iron, malabsorption of vitamins. Symptoms includes pale skin, fatigue, palpitations, shortness of breath.

Progressive changes in fossils of different ages provides one of the strongest lines of evidence for

Answers

Are there any options you can provide?

A bakery makes 640 muffins every day. Which equation shows how to find the number of muffins the bakery makes in a week?

Answers

X=7(640) // X being the number of muffins made in a week, 7 being the number of days in a week, and 640 being the number of muffins made each day.

A(n) _____________ is a pattern of dark bands on photographic film that is made when DNA fragments are separated by gel electrophoresis and tagged. The photograph produced is often used to determine whether or not suspects were involved in a crime.

Answers

A DNA Fingerprint is a pattern of dark bands on photographic film that is made when DNA fragments are separated by Gel electrophoresis and tagged. Every human has their own unique fingerprints which will leave its trace to whatever objects we touched and our Law enforce held the database of all legal citizen's fingerprints

Answer:

DNA fingerprint

Explanation:

DNA fingerprinting is a technique widely used in criminal and forensic investigation. This technique aims to identify fragments of DNA, which like a fingerprint can reveal someone's identity. This is because each individual has a unique, particular and individual ANA, even the restriction zones in the DNAs are different between two different individuals.

This technique is possible because a DNA is subject to the action of restriction enzymes. When biological material is found at a crime scene, the DNA of that material is subjected to restriction enzymes that fragment the molecule into different portions. These portions can be viewed through X-rays, if marked with a radioactive marker. After this marking, the DNA fragments are subjected to agarose gel electrophoresis, revealing a kind of "barcode" that is specific to a single person in the world.

Approximately how many species have been identified on earth?

Answers

about 1.7 million species have been identified on earth.
Final answer:

Approximately 1.2 million species have been identified on Earth, which includes various forms of life. However, the actual number of species on Earth is believed to be considerably higher.

Explanation:

Approximately, scientists have identified around 1.2 million species on Earth. This count involves various forms of life, including plants, animals, fungi, and micro-organisms. However, it is important to note that this figure represents only a fraction of the actual number of species believed to exist; some estimates suggest that there might be anywhere from 8.7 million to 1 trillion different species on Earth, many of which have not been discovered or described yet.

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Antipsychotic drugs generally refer to drugs used to treat __________.

Answers

bipolar disorder to control psychotic symptoms such as hallucinations, delusions, or mania symptoms. These symptoms may occur during acutemania or severedepression

The ________ of the eye works similarly to the shutter of a camera

Answers

Toenail?

Correct me if I'm wrong.

For a parasitic organism such as ascaris what would be the selective advantage of a cuticle

Answers

Ascaris is a type of parasitic nematode worm that inhabits the small intestine of its host, which includes humans. The intestine can be a harsh environment for biological material as it is alkaline and contains enzymes to facilitate the break down and absorption of nutrients. The presence of a cuticle can act as a physical barrier, protecting the parasite from these elements. 

What type of plant has no vascular tissue daisy fern moss or pine tree

Answers

moss plants do not have vascular tissue.
hope i helped :D
Moss for sure

Hope this helps

~ Jordan ~

Of what morphology are most bacteria that possess flagella

Answers

Mostly bacillus (bacteria with cylindrical/rod shaped morphology) I'm pretty sure, you don't see any type of coccus (bacteria with spherical morphology) with flagella.

"tertiary structure of a protein is maintained by (list the interactions)"

Answers

Protein tertiary structures are known to be a three dimensional structure of a protein with a single polypeptide chain (backbone) and one or more protein secondary structures known as protein domains.

Tertiary Structure Interactions

1) Hydrophobic Interactions: they are non- covalent bonds and very important in the formation of tertiary structure. 

2) Ionic Bonds: the interaction of both positive and negative amino acids forms a bond that helps to stabilize the protein molecules. 

3) Hydrogen Bonds: this bond exit between the amino acid with hydrophilic side chain found on the surface of the molecules and water molecules in a solution.

4) Disulfide Bridges: it is a strong covalent bond commonly found between cysteine residues in close proximity space. 

The nurse is preparing discharge teaching for a client with a history of recurrent pneumonia. what deep breathing techniques will the nurse plan to teach?

Answers

Deep breathing techniques help the lungs to recover from the loss of its capacity. This technique moves the air down to the bottom areas of the lungs, helps open air passages, and moves mucous out (which can also be facilitated through coughing). This process improves blood and oxygen supply to your lungs.

Where should the breastplate on the restraint straps rest in the infant?

Answers

For the answer to the question above, the best part to place the breastplate to restraint an infant especially on cars in the armpit level. If you place it below. They might have a difficulty in breathing. Higher than armi. they might choke their neck. So the best place is in the Armpit level.

Answer:

Armpit level

Explanation:

To ensure the safety of the whole family it is important that all members are properly protected inside the car. This protection is done through the seat belt, however, in the case of babies and very young children the seat belt does not promote safety, requiring different accessories to protect the little ones. In the case of infants it is important that restraint straps are used so that the breastplate is located at the level of the axolas, in the child.

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Can anyone help me answer these Grade 8 Math questions? plz put graph form 1) 2x - y = -13 y = x + 9 2)3x + 2y = 10 6x - y = 10 3)4x - 3y = 5 3x + 2y = -9 4)x + y = 7 x - y = -1 Which of the following rational numbers is equal to 3.4? (line above the four) A: 31/9B. 32/9C. 33/9D. 34/9 I confused on how to solve these trigonometric ratios. Which country in Southern Europe provides the best harbors? The "three es" of injury preventioneducation, enforcement, and engineeringwere first applied in which industry? how many syllables are there for each line? Plz help ASAP! Which of the following most likely functions as the falling action in a story? Graduation day was rainy, sticky, and uncomfortable, just like the school gym on any given day. I had been waiting for this day to come for almost the entire school year. We gathered, as usual, under the spreading oak tree on top of the hill behind the school. We never knew all the trouble our adventure caused for Dad until many years later. 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He spent only one night in jail, however, as a relative paid the tax for him. He was reportedly furious that any tax was paid on his behalf. It was this experience that Thoreau wrote about in an essay called "Civil Disobedience." In this essay, he argued that being moral and just came before allegiance to government. He wrote If the machine of government is of such a nature that it requires you to be the agent of injustice to another, then, I say, break the law." He also felt that voting was not enough to ensure that the right thing be done. He wrote that "even voting for the right is doing nothing for it A wise man will not leave the right to the mercy of chance" He felt that one had a moral responsibility to resist unjust laws. Which line from Part 1 best explains Thoreau's message? 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What type of reproduction shuffles the genes how could you use the conversion table on page 618 to convert 700 cenimeters to meters