The human insulin gene contains a number of sequences that are removed in the processing of the mRNA transcript. In spite of the fact that bacterial cells cannot excise these sequences from mRNA transcripts, explain how a gene like this can be cloned into a bacterial cell and produce insulin.

Answers

Answer 1

Answer:

In order to produce human insulin in a bacterium you need to isolate the mature mRNA (after the excision of the introns), produce a DNA copy using the enzyme retrotranscriptase, clone the gene in an expression vector and transform bacterial cells with this genetic construct to produce the insulin.

Explanation:

Eukaryotic cells perform a process called splicing, in which introns (the non-coding fragments of the gene). are excised from the mRNA Prokaryotic cells (i.e. bacteria) don’t perform this process. So, in order to produce a recombinant protein in a bacterium, insulin in this case, the best strategy should be to produce a complementary DNA (cDNA) of the mature mRNA (AFTER the splicing process, the excising of the introns).


Related Questions

What biomes spans the largest annual mean temperature range

Answers

Answer: Desert spans the annual mean temperature range.

Explanation:

Biomes are large ecological community of plant and animal on the earth surface that have characteristics for the environment they live in. A desert is an example of biomes. It is a barren area of land where there is high temperature and low precipitation (rainfall). It is a place where living conditions is difficult. Lact of vegetation exposed the areato direct sunlight and there is increased temperature. Desert are formed by weathering process I.e the breakdown of rocks into smaller particles through the activities of some agent like water or wind.

Assume that you are working with a mutant, nutritionally deficient strain of Escherichia coli and that you isolate "revertants," which are nutritional-normal. Describe, at the molecular level, two possible causes for the "reversion to wild type."

Answers

Answer:

Mutation may be defined as the sudden heritable change in the genetic sequence of the organism. The mutation can cause the characteristics phenotype in the individual.

Two ways by which the reversion to wild type occur are true reversion and suppression mutation. The true reversion process involves the precision of the correction of the original mutation. The suppression intragenic or intergenic  mutation introduce the changes in the mutant gene and compensate for the wild type product.

Final answer:

At the molecular level, reversion to wild-type in nutritionally deficient E. coli can occur through a back mutation, restoring the ability to produce a necessary nutrient, or a suppressor mutation, which bypasses the block caused by the original mutation.

Explanation:

When working with a mutant, nutritionally deficient strain of Escherichia coli and isolating revertants that are nutritionally normal, two possible molecular causes for the reversion to wild-type are back mutation or suppressor mutation.

Firstly, a back mutation could occur, which is a second mutation that restores the original DNA sequence or results in a new sequence that somehow compensates for the effect of the original mutation, allowing the E. coli to revert to being able to synthesize the nutrient it was previously unable to produce.

Secondly, a suppressor mutation may arise in a different location within the genome that counteracts the original mutation's effect. This does not reverse the original mutation but provides a new pathway or enzymatic function that bypasses the block created by the original mutation, allowing the cell to grow without the required nutrient supplement.

Galápagos medium ground finches are found on Santa Cruz and San Cristóbal islands, which are separated by about 100 km of ocean. Occasionally, individuals from either island fly to the other island to stay. This can alter the allele frequencies of the population through which of the following mechanisms?
Natural selection
Genetic drift
Gene flow
Mutation

Answers

Answer:

Gene flow

Explanation:

Gene flow, also known as migration, gene migration or allele flow, is the movement of genetic material when individuals move from one place to another, this migration generates a modification in the allele frequencies combining the gene pool between both populations. This is exactly what is happening in the example given.

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During the major response stages of acute radiation syndrome after the prodromal stage, the period when symptoms that affect the hematopoietic, gastrointestinal, and cerebrovascular systems become visible, is called

Answers

Manifest illness stage is the period when symptoms that affect the hematopoietic, gastrointestinal, and cerebrovascular systems become visible during the major response stages of acute radiation syndrome after the prodromal stage.

Explanation:

Acute radiation syndrome (ARS) due to radiation toxicity affects the parenchymal cells of specific tissues. According to the tissue system affected, the ARS typically presents either as bone marrow (hematopoietic), gastrointestinal, or cardiovascular syndrome.

The four major response stages of acute radiation syndrome are – Prodromal, latent, manifest illness, recovery or death.

Although the stage next to prodromal stage is latent stage, the symptoms manifests itself during the manifest illness stage. The manifestation, severity and recovery of symptoms vary with each syndrome.

Symptom manifestation during manifest illness stage

Hematopoeitic ARS – drop in blood cell count, hemorrhage, anorexia, malaise

Gastrointestinal ARS – anorexia, diarrhea, dehydration, fever, electrolyte imbalance

Cardiovascular/central nervous system ARS – convulsions, coma

Vectors such as pUC18 and others of the pUC series contain a large number of restriction enzyme sites clustered in one region. Which term describes this advantageous arrangement of restriction sites?

Answers

Answer:

multiple cloning site

Explanation:

Based on the information provided within the question it can be said that the term being described is a multiple cloning site or MCS for short. Like mentioned in the question this refers to a region of DNA located inside a Plasmid, which holds various Restriction enzyme sites clustered in one reagion, each of which are unique.

Which of the following researchers are correctly matched with their work?
A.Erwin Chargaff --- Demonstrated the assimilation of external DNA into a cell; mixing a heat-killed pathogenic strain of bacteria with a living nonpathogenic strain can convert some of the living cells into the pathogenic form
B.Alfred Hershey and Martha Chase ---- Showed that the DNA, but not the protein, entered the bacterial cells, and was therefore concluded to be the genetic materialE.James Watson and Fredrick Crick --- Determined the structure of DNA, an elegant double-helical model
C.Frederick Griffith --- X-ray diffraction of crystallized DNA, suggested helical structure and diameter
D.Rosalind Franklin --- Analysis of the relative base composition of DNA

Answers

Answer:

The answer is letter B

Explanation:

B.Alfred Hershey and Martha Chase ---- Showed that the DNA, but not the protein, entered the bacterial cells, and was therefore concluded to be the genetic materialE.James Watson and Fredrick Crick --- Determined the structure of DNA, an elegant double-helical model

Rosalin Franklin - Xray diffraction of crystallized DNA, that is why letter C is incorrect.

Frederic Griffith- Demonstrated the assimilation of external DNA into a cell; mixing a heat-killed pathogenic strain of bacteria with a living nonpathogenic strain can convert some of the living cells into the pathogenic form, that is why letters A and C are incorrect.

Fats in foods a. add taste and contribute to "mouth feel" b. are digested and absorbed in the stomach c. carry water-soluble nutrients d. need to be eliminated to have a healthful diet

Answers

Answer:

The correct answer is a. add taste and contribute to "mouth feel".

Explanation:

Fats in foods contribute to mouthfeel, meaning that the mouth literally feels the food coming in. It differs from the taste, but generally has similar effects on the brain.

Which statement made by the patient indicates to the nurse that understanding about discharge instructions on antihyperlipidemic medications has occurred?
a. "Antihyperlipidemic medications will replace the other interventions I have been doing to try to decrease my cholesterol."
b. "It is important to double my dose if I miss one in order to maintain therapeutic blood levels."
c. "I will stop taking the medication if it causes nausea and vomiting."
d. "I will continue my exercise program to help increase my high-density lipoprotein serum levels."

Answers

Answer:

The correct answer is d i will continue my exercise programme to help increase my high density lipoprotein serum levels.

Explanation:

Antihyperlipidemic drug is pescribed to patient suffering from high plasma cholesterol.High plasma cholesterol level is much dangerous for our body because it can leads to the development of coronary artery disease.

    When discharge instruction on hyperlipidemic medication is given to a patient at that time patient is informed to continue his/her exercise program me to maintain a high level of good cholesterol also known as high density lipoprotein.

A primigravid client at 24 weeks’ gestation has received permission from the primary care provider to make a 6-hour automobile trip to visit her parents. After the nurse teaches the client about precautions to take during the trip, which client statement indicates the need for further instruction?

Answers

Final answer:

The importance of a pregnant woman taking frequent breaks to walk around on a long trip is to reduce the risk of deep vein thrombosis (DVT). A statement indicating misunderstanding of this precaution would show the need for further instruction on travel safety for pregnant women.

Explanation:

The question pertains to a primigravid client at 24 weeks’ gestation who has planned a 6-hour automobile trip and needs to know the precautions to take. During long trips, pregnant women are advised to take several precautions to maintain their health and safety as well as that of their unborn child. Key advice includes taking frequent breaks to walk around and reduce the risk of blood clots, staying hydrated, wearing a seat belt properly, and avoiding driving for long periods without rest.

A statement indicating the need for further instruction would be if the client said, "I won't need to stop for breaks; I can sit still the whole trip." This statement shows a misunderstanding of the need to reduce the risk of deep vein thrombosis (DVT) by moving around periodically. The proper use of a seat belt, positioned under the belly and across the chest, is also vital for safety during pregnancy.

The correct option indicating the need for further instruction is B) "I will sleep for 1 hour at the halfway point of the trip." Sleeping for a prolonged period during a car trip poses risks of blood clots and accidents. The other options emphasize appropriate precautions such as wearing a seat belt, staying hydrated, and taking regular breaks.

The correct answer indicating the need for further instruction is B) "I will sleep for 1 hour at the halfway point of the trip." While rest is important during a long journey, especially for a pregnant woman, sleeping for a prolonged period, especially during a car trip, poses risks. This statement raises concerns about the safety of the mother and the unborn baby.

Firstly, prolonged periods of sitting, especially in a sedentary position like driving, can increase the risk of blood clots, particularly in pregnant women who already have an increased risk due to changes in blood circulation during pregnancy. Secondly, falling asleep while driving is extremely dangerous and can lead to accidents, endangering not only the mother and her unborn child but also other passengers and drivers on the road.

The other options demonstrate appropriate precautions:

A) "I will be sure to wear the car seat belt while traveling." - This is a crucial safety measure to protect both the mother and the baby in case of an accident. However, the seat belt should be worn properly, with the lap belt positioned under the abdomen and the shoulder belt between the breasts and to the side of the belly.

C) "I will drink plenty of fluids to avoid dehydration." - Staying hydrated is important during pregnancy, especially during long trips, to prevent dehydration and maintain overall health.

D) "I will take frequent rest breaks every 2 hours." - Taking regular breaks during long car trips is essential to stretch the legs, improve circulation, and reduce the risk of blood clots. It also allows the pregnant woman to walk around and relieve pressure on her back and legs, which can become uncomfortable during extended periods of sitting.

While rest is important during a long journey, advising the pregnant woman to sleep for a prolonged period during a car trip poses significant risks to her and her unborn child. Instead, emphasis should be placed on taking regular breaks, staying hydrated, and wearing the seat belt correctly to ensure the safety and well-being of both the mother and the baby.

Complete Question:- A primigravid client at 24 weeks’ gestation has received permission from the primary care provider to make a 6-hour automobile trip to visit her parents. After the nurse teaches the client about precautions to take during the trip, which client statement indicates the need for further instruction?

A) "I will be sure to wear the car seat belt while traveling."

B) "I will sleep for 1 hour at the halfway point of the trip."

C) "I will drink plenty of fluids to avoid dehydration."

D) "I will take frequent rest breaks every 2 hours."

How many cusps are located on the right atrioventricular valve

Answers

Answer:

3

Explanation:

these are are located between the right upper atrium and lower right ventricle. These cusps are

1.Anterior

2. Septal

3. Posterior.

Anatomically, the base of each cusp is held firmly or anchored by fibrous structure called CHORDAE TENDINEAE.

Thier functions is to pevent regurgitation of the blood and the wall of the right ventricle into the atrium during ventricular systole; contraction of the ventricle.Therefore they blocked the passage and ensured blood flows into the pulmonary arteries to reach the lungs.

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An estuary collects sediments from the ocean and rivers that feed into it. The sediments swirl around and then settle to form a mudflat. Eel grass is then established on the mudflat. The ecosystem changes over time and ultimately develops into a salt marsh that contains mangrove trees.

Which of the following is likely NOT involved in this example of ecological succession?

A)
The rotting remains of plants add to the fertility of the soil.


B)
The soil becomes so fertile that eel grass is replaced by other plant species.


C)
The roots from plants help stabilize the sediment, keeping it in place.


D)
The concentration of salt becomes so high that all plant life is destroyed.

Answers

Answer:

Explanation: d

NOT involved in this example of ecological succession where the salt concentration becomes so high that all plant life is destroyed.

What is the definition of an estuary?

Estuaries are semi-enclosed coastal bodies of water that have a free connection with the sea and in which seawater is measurably diluted with fresh water from land drainage.

With this information, we can conclude that you are NOT involved in this example of ecological succession where the concentration of salt becomes so high that all plant life is destroyed.

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Bees can see colors we cannot see, and they can detect minute amounts of chemicals we cannot sense. Unlike many insects, bees cannot hear very well. A biologist would probably give which of the following as the ultimate explanation for their poor hearing?a.) If a bee could hear, its highly programmed brain would be overwhelmed with information.b.) If bees could hear, the noise of the hive would be overstimulating.c.) This is an example of altruism.d.) Bees are too small to have functional ears.e.) Hearing may not contribute much to a bee's reproductive success.

Answers

Answer: The answer would likely be E.

Explanation:

The biologist would likely explain this concept from the viewpoint of evolution. As hearing does not contribute to the bee's reproductive success, enhanced hearing traits in bees were not selected for and hence bees' hearing ability was not an adaptation that enhanced its reproductive success and bees cannot hear very well.

A viewer closely focused on a complex task, such as being asked to count how many times a group of people threw a ball back and forth, may miss other nonattended stimuli like dancers moving through the group of people due to an attentional phenomenon called____________

Answers

Answer:

Inattentional blindness

Explanation:

Inattentional blindness is a phenomenon which occurs as a failure of an individual to take notice of an object or event that is very obvious due to the fact that such objects or event were not expected, rather attention was fully focused elsewhere on another object, task or event. This type of blindness occurs simply as a result of lack of attention of an individual on events, objects or tasks that are not expected, while attention is focused on expected stimuli. It is not as a result of defect in vision. One tends to miss an obvious and clear event, object or task, unless one focused one’s attention on such object.

A human baby performs a sucking behavior perfectly when it is put in the presence of the nipple of its mother's breast. This is an example of____________.

Answers

Answer:

The answer is innate behavior.

Explanation:

A human baby performs a sucking behavior perfectly when it is put in the presence of the nipple of its mother's breast. This is an example of__innate behavior__________.

According to the acid-growth hypothesis, auxin causes increased acidity in cell walls, which then activates ____ that disrupt(s) bonds between cellulose microfibrils, allowing cell expansion

Answers

Answer:

proteins called expansins

Explanation:

The complete question is :

According to the acid-growth hypothesis, auxin causes increased acidity in cell walls, which then activates ____ that disrupt(s) bonds between cellulose microfibrils, allowing cell expansion.

a. a membrane potential

b. an mRNA

c. AUX1 transporters

d. K+ ions

e. proteins called expansins

The acid growth hypothesis explains the growth of cells and organs in the plants. According to this hypothesis, the plant hormone auxin causes induction of the H+ proton extrusion into the apoplast which leads to the activation of expansin which a pH-sensitive protein.Expansin is responsible for wall stretching and relaxation and causes hydrolysis of hydrogen bonds and thus helps in cell expansion.

The range of cattle egrets has expanded between 1937 and today. How would an ecologist likely explain the expansion of the cattle egret?
A) the first egrets to colonize South America evolved into a new species capable of competing with the native species of herons and egrets
B) A habitat left unoccupied by native herons and egrets met the biotic and abiotic requirements of the cattle egret transplants and their descendants
C) There are no predators for cattle egrets in the New World, so they continue to expand their range

Answers

A habitat left unoccupied by native herons and egrets met the biotic and abiotic requirements of the cattle egret transplants and their descendants  

Answer: Option B

Explanation:

The cattle egret was a species of Heron to be first seen in Greater Caribbean Basin. They acquired their name “Cattle egret” as they were found to be co-existing with the grazing cattle and feeding on the insects that come out during grazing and also feed on the ticks present on the cattle’s body.

The cattle egret within a span of few years showed a dramatic increase in its population. This was attributed by their high reproduction rate leading to their extensive population growth. They also do not have any predators devouring them. They also feed on anything and everything to survive be it insects, rodents or small reptiles. They were good flyers and they were also able to get accustomed to their surroundings and reproduce.

Final answer:

An ecologist would attribute the cattle egret's range expansion to the species finding suitable biotic and abiotic conditions in new habitats, the role of human activities in their spread, and possibly the introduction of new genotypes that thrive in different conditions. (Option B)

Explanation:

An ecologist would likely explain the expansion of the cattle egret's range by considering factors such as habitat suitability and the role of human activities in spreading species. Option B describes a situation in which cattle egrets found suitable biotic and abiotic conditions in new areas unoccupied by native herons and egrets, enabling them to establish and expand their population. Human transportation of goods and intentional organism trade have facilitated species introduction into new ecosystems, often providing them with environments lacking natural predators, allowing these species to thrive and spread further.

Moreover, the introduction of new genotypes to an existing population of a species can result in a sudden range expansion if these genotypes are better adapted to the local conditions. This has been seen in cases like the brown anole in Florida or the European green crab, where new genotypes introduced into the population contributed to their successful spread.

According to the "wobble hypothesis," what is the identity of the tRNA that can recognize the following codons: CAU, CAC, and CAA? According to the "wobble hypothesis," what is the identity of the tRNA that can recognize the following codons: CAU, CAC, and CAA? 3'-IUG-5' 3'-GTI-5' 3'-GUI-5' 3'-III-5'

Answers

Explanation:

The wobble hypothesis states that the first two bases within a codon for an amino acid have an exact pairing with anticodons of tRNA. However, the pairing of the third bases may vary or wobble- thus a tRNA can recognise multiple codons.

3'-GUI-5'  can recognise  CAU, CAC, and CAA

wobbling occurs at the 5' end I represents the wobble anticodonpurines pair up with pyrimidines in base pairs

Further Explanation:

The nucleic acids are comprised of smaller units called nucleotides and function as storage for the body’s genetic information. These monomers include ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). They differ from other macromolecules since they don’t provide the body with energy. They exist solely to encode and protein synthesis.

Basic makeup: C, H, O, P; they contain phosphate group 5 carbon sugar does nitrogen bases which may contain single to double bond ring.

Codons are three nucleotide bases encoding coding and amino acid or signal at the beginning or end of protein synthesis.

RNA codons determine certain amino acids so the order in which the bases occur within in the codon sequence designates which amino acid is to be made bus with the four RNA nucleotides (Adenine, Cysteine and Uracil) Up to 64 codons (with 3 as stop codons) determine amino acid synthesis. The stop codons ( UAG UGA UAA) terminate amino acid/ protein synthesis while the start codon AUG begins protein synthesis.

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The trees below present a hypothetical situation where "T" represents the evolution of a male trait that is attractive to females and "P" represents where the preference in females evolved. Which tree below is consistent with the sensory bias hypothesis?

Answers

Answer:

P

Explanation:

The main conclusions  of sensory bias are that natural selection is the major evolutionary mechanism that affects preference and that sexual selection on preferences is quantitatively negligible. this explain why females exhibit preference.

__________ is a progressive degenerative disease of the basal nuclei that affects the dopamine-secreting pathways.

a.Parkinson's disease
b.mad cow disease
c.Huntington's disease
d.Alzheimer's disease

Answers

Answer:

a.Parkinson's disease

Explanation:

Dopamine is a neurotransmitter who's function is to be the body's reward center "happy hormone" and it is also involved in motor control.

For unknown reasons in the elderly, dopamine is lost when the dopamine-producing nerve cells die in the basal ganglia and substanta nigra which results in movement problems such as, tremor, slowness of movement, stiffness, and balance problems.

This disease is called Parkinsons disease which is a progressive and degenerative disorder

Levels of total cholesterol and LDL cholesterol are directly related to the frequency of coronary heart disease.a) true b) false

Answers

Answer:

a) true

Explanation:

Blood conducts cholesterol from the intestine or liver to the organs that need it and does so by binding to particles called lipoproteins. There are two types of lipoproteins:

Low density (LDL): they are responsible for transporting new cholesterol from the liver to all the cells of our body.

High density (HDL): collect unused cholesterol and return it to the liver for storage or excretion abroad through bile.

According to this interaction we can talk about two types of cholesterol:

Bad cholesterol: Cholesterol when attached to the LDL particle is deposited in the wall of the arteries and forms atheroma plaques.

Good cholesterol: Cholesterol when attached to the HDL particle transports excess cholesterol back to the liver for destruction.

- If their blood levels rise they produce hypercholesterolemia. It is shown that people with blood cholesterol levels of 240 have twice the risk of having a myocardial infarction than those with figures of 200.

- When the cells are unable to absorb all the cholesterol that circulates in the blood, the excess is deposited in the wall of the artery and contributes to its progressive narrowing causing atherosclerosis.

- If an atheromatosis patient keeps his blood cholesterol levels very low, he can get that cholesterol from the arterial wall back to the blood and be eliminated there. Therefore, patients who have suffered myocardial infarction or brain accident are recommended to maintain very low cholesterol levels to try to clean their arteries.

Hypercholesterolemia has no symptoms or physical signs, so its diagnosis can only be made by a blood test that determines cholesterol levels and also triglycerides. It is convenient that people at risk of having a dyslipidemia (alteration of the normal levels of these fats), who have relatives with ischemic heart disease and other cardiovascular diseases, undergo this test from an early age.

Final answer:

It is true that high levels of total cholesterol and LDL cholesterol are directly related to an increased risk of coronary heart disease. LDL, or 'bad' cholesterol, contributes to the buildup of plaque in arteries, leading to atherosclerosis and coronary artery disease, while HDL, the 'good' cholesterol, helps reduce heart disease risk.

Explanation:

Levels of total cholesterol and LDL cholesterol being directly related to the frequency of coronary heart disease is indeed true. Research shows that low-density lipoproteins (LDL cholesterol), often termed as “bad” cholesterol, facilitate the build-up of plaque in the arteries—a process known as atherosclerosis. This can lead to coronary artery disease, where the coronary arteries become narrowed or blocked, potentially resulting in heart attacks. Conversely, high-density lipoproteins (HDL cholesterol), or “good” cholesterol, help in reducing the risk of heart disease by transporting surplus cholesterol back to the liver.

Furthermore, lifestyle factors such as unhealthy eating habits, lack of physical activity, and smoking can exacerbate the increase in LDL cholesterol and decrease in HDL cholesterol. To maintain heart health, it is advised to keep the LDL levels under 100 mg/dL and the HDL levels above 40 mg/dL for men and 50 mg/dL for women. Regular exercise, a healthy diet, and maintaining a healthy weight are essential in controlling cholesterol levels and reducing the risk of cardiovascular diseases.

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Which of the following animals indicated in the Antarctica Food Web folder utilizes the Crabeater Seal as a source of food? a. Humpback Whale b. Sharks c. Krill d. Leopard Seal e. Squid

Answers

The animal that uses the Crabeater Seal as a food source in the Antarctica Food Web is the Leopard Seal. Humpback whales, sharks, krill, and squid do not feed on crabeater seals. As for the effects of melting sea ice in Alaska, polar bears would be the most affected.

The correct answer to the question of which animal in the Antarctica Food Web utilizes the Crabeater Seal as a source of food is d. Leopard Seal. Leopard seals are top predators in the Antarctic ecosystem, known to prey on other marine mammals, including the crabeater seal. This information helps us understand the dynamics of Antarctic food webs and the importance of each species in maintaining ecological balance.

Humpback whales primarily feed on krill and small fish, not on crabeater seals.

Sharks are not typically part of the Antarctic food web where crabeater seals live.

Krill are a primary food source for many animals, but they do not consume crabeater seals.

Squid also do not feed on crabeater seals, as they have different dietary preferences.

Concerning the Arctic food web and the impact of sea ice melting in Alaska, polar bears would be most affected (option b) because they rely on sea ice to hunt for seals, their primary food source.

Which example describes biological evolution?
a.Over time, a caterpillar species becomes as green as the leaves it eats.
b.The average woman's dress size has increased over the last 50 years.
c.You are vaccinated against polio and are protected against the disease.
d.A tree branch is broken off and another grows in its place.

Answers

Answer:

a.Over time, a caterpillar species becomes as green as the leaves it eats.

Explanation:

Evolution means the gradual formation of complex organisms from simple types, a process that usually take several years (geological time).

The gradual change happens as a result of continuous adaptation by organisms to ever changing environment.

Option b could have been considered along with option a, but evolution process takes much more than 50 years.

Hence, the correct option is a.

As a first step in the proposal, the investigator(Wouldbe Fiancé) needs to make many copies of DNA of known sequence. How would they do that? A)Carry out DNA cloning of the desired sequence into a plasmid vector and then put that into bacteria and wait for the bacteria to copy the gene every time the cells divide. B)Wait for helicase to unzip the genes. C)Make a long sequence of adenines so they could get paired with U. D)Alterthe reading frame of the mRNA.

Answers

Answer:

The correct option is A) Carry out DNA cloning of the desired sequence into a plasmid vector and then put that into bacteria and wait for the bacteria to copy the gene every time the cells divide.

Explanation:

The DNA can be easily cloned by the help of vectors like plasmids. Plasmids can be described as an extra-chromosomal ring which is present in bacteria. The plasmid has many multiple cloning sites where the desired DNA can be incorporated. the plasmid with the foreign DNA is then incorporated back into a bacterial cell. As bacterial cells like E.coli divide in a short period of time, many copied of our DNA could be obtained whenever the bacterial cell replicates.

The short narrow tube that runs from the auricle to the tympanic membrane

Answers

Answer:

External auditory meatus

Explanation:

The pinna leads into the external auditory meatus which is a tube that directs sound waves to the tympanic membrane. The tube is lined with hairs which trap solid particles that may enter the ear. It is also lined by wax secreting cells whose function is to secrete wax that traps dust and prevents entry of solid particles. The wax also maintains the flexibility of the eardrum.

A 28-year-old presets to the ER reporting severe chest pain that worsens with respirations or lying down. Other signs include a fever, tachycardia, and a friction rub. Assessment findings support which medical diagnosis?A. Acute pericarditis.B. Myocardial infarction (MI).C. Stable angina.D. Pericardial effusion.

Answers

Answer:

The answer is letter A

Explanation:

Acute pericarditis.  Because this inflamation causes chest pain, pericardial friction rub, fever. It is often associated to viral infecctions , but it may also be caused by other diseases and drug use.

A small town in the piney woods of East Texas has a soccer field composed of native grasses. The soccer field is mowed once a week.

What effect does continual mowing have on the ecology of the field?

A)
Mowing increases the likelihood of nonnative species displacing native species.


B)
Mowing increases the number of species found in the field.


C)
Mowing causes different types of communities to form across the field.


D)
Mowing maintains a low species diversity by inhibiting succession.

Answers

Explanation:

D)  Mowing maintains a low species diversity by inhibiting succession.

Mowing reduces the chances of growth of a new species within the community that may add to its complexity.

Succession involves small scale changes within a community that occur repeatedly over the life of that community.  Ecological communities are highly dynamic- they gradually evolve. Typically their progression involves:

colonizing species exploiting uninhabited areas (Primary succession) becoming a habitable and increasingly complex community there is increased diversity of organisms (Secondary)

Further Explanation:

The makeup of biological communities is crucial to defining Primary and Secondary succession; eventually, through changes in this makeup, a steady-state or equilibrium is reached called a climax community. While Primary succession starts off with the modification of a previously unoccupied area along with increasing variation; secondary succession begins after major disruption in the community such as fires, storms and flooding.

However, the process of becoming a climax community can be pretty complicated- it is very dependent on other factors like temperature and rainfall. Communities that experience rapid change, frequent major disruptions and even human intervention, are less likely to attain a stable equilibrium and may never become climax communities.

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Movement of the chromosomes during anaphase would be most affected by a drug that prevents mitosis and cell division. What is this drug?

Answers

Answer:

Colchicine

Explanation:

Colchicine is an alkaloid derived from the autumn crocus (Colchicum autumnale).

The effect of colchicine, which inhibits microtubule polymerization and thus assembly of the mitotic spindle, demonstrates the presence of another checkpoint in the cell cycle. When colchicine is added to cultured cells, the cells enter mitosis and arrest with condensed chromosomes. With increasing time, a large fraction of the cells in a culture become arrested, thus permitting determination of the size, shape, and number of mitotic chromosomes — that is, the karyotype  — in multiple cells. A checkpoint control somehow senses when the mitotic spindle has not assembled properly and prevents activation of the APC polyubiquitination system that normally leads to degradation of the anaphase inhibitor, required for onset of anaphase, and later to the degradation of mitotic cyclins, required for the exit from mitosis. As a result, MPF activity remains high, chromosomes remain condensed, and the nuclear envelope does not re-form.

Fatty acids usually have an even number of carbons in their structures. They are catabolized by a process called beta-oxidation. The end products of the metabolic pathway are acetyl groups of acetyl CoA molecules. These acetyl groups _____.

A) directly enter the electron transport chain
B) directly enter the energy-yielding stages of glycolysis
C) are directly decarboxylated by pyruvate dehydrogenase
D) directly enter the citric acid cycle

Answers

Answer:

D. Directly enter the citric acid cycle

Explanation:

In biochemistry and metabolism, beta-oxidation is the catabolic process by which fatty acid molecules are broken down in the cytosol in prokaryotes and in the mitochondria in eukaryotes to generate acetyl-CoA, which enters the citric acid cycle, and NADH and FADH2, which are co-enzymes used in the electron transport chain. The NADH and FADH2 produced by both beta oxidation and the TCA cycle are used by the mitochondrial electron transport chain to produce ATP. Complete oxidation of one palmitate molecule (fatty acid containing 16 carbons) generates 129 ATP molecules.

The acetyl groups from the catabolization of fatty acids via beta-oxidation directly enter the citric acid cycle. This is where the acetyl-CoA is further metabolized, contributing to the production of ATP, NADH, and FADH2.

The end products of the metabolic pathway of beta-oxidation in which fatty acids are broken down are acetyl groups of acetyl-CoA molecules. These acetyl groups directly enter the citric acid cycle (also known as the Krebs cycle). The citric acid cycle is a series of chemical reactions used by all aerobic organisms to generate energy. The acetyl-CoA combines with oxaloacetate to form citrate, which then undergoes a series of reactions releasing energy and producing carbon dioxide, NADH, and FADH2, which are utilized by the electron transport chain.

The process of beta-oxidation of fatty acids takes place within the mitochondria and is a significant contributor to the production of ATP when glucose levels are low. After being catabolized to acetyl-CoA, these molecules can't directly enter the electron transport chain or glycolysis, nor are they immediately decarboxylated by pyruvate dehydrogenase; rather, they proceed to the citric acid cycle for further metabolism.

How do lichens contribute to primary succession? Lichens begin to break down rock to form soil. Lichens decompose organic matter from animals and plants. Lichens are nitrogen-fixing bacteria. Lichens convert carbohydrates into fossil fuels.

Answers

Final answer:

Lichens contribute to primary succession by breaking down rocks, forming soil, and adding organic material. They are a mutualistic association between a fungus and a photosynthetic partner, with each partner providing necessary components for survival and growth. Some cyanobacteria in lichens additionally fix nitrogen.

Explanation:

Lichens contribute to primary succession by colonizing rock surfaces in otherwise lifeless environments, breaking down rocks to form soil, and adding organic material to further contribute to soil formation. Lichens are a mutualistic association between a fungus and a photosynthetic partner, such as an alga or a cyanobacterium. The fungus supplies minerals and protection, while the photosynthetic partner provides carbon and energy. Some cyanobacteria in lichens also fix nitrogen from the atmosphere, contributing nitrogenous compounds to the association.

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Lichens are pivotal in primary succession, colonizing barren rock surfaces and initiating soil formation through the breakdown of rock and nitrogen fixation.

Lichens contribute to primary succession by acting as pioneer species that colonize lifeless rock surfaces, such as those left after glacial recession or volcanic activity. Through a symbiotic relationship between a fungus and either an alga or a cyanobacterium, lichens are formidable survivors that can thrive without soil. They produce acids that help break down rocks to form the first layers of soil. Moreover, certain types of lichens are capable of nitrogen fixation, which adds essential nutrients to the forming soil, supporting future growth of plant life.

Which of the following actual (i.e., real) products of biological activity is not likely to be a source of the increases in greenhouse gases observed in recent history? A. carbon dioxide produced as a result of aerobic cellular respiration in living organisms B. carbon dioxide produced by use of fossil fuels in automobiles, heating and cooling, and industry C. increases in the amount of water vapor the atmosphere can hold (or relative humidity) at higher global temperatures

Answers

Answer:

A)

Explanation:

Carbon dioxide produced as a result of aerobic cellular respiration in living organisms.

Is not B, because the carbon dioxide produced by the use of fossil fuels is not use inside a greenhouse.

And the amount of water vapor is not a source for CO2

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