In a gene cloning experiment, why are white colonies desirable (rather than blue colonies)?

Answers

Answer 1

This is because the white colonies are the ones that have taken up the recombinant DNA, while the blue ones have not. This is called blue-white screening, a molecular cloning technique, and is conducted on X-gal medium and utilized selection using the beta-galactosidase gene.


Related Questions

An organism has the following characteristics cell wall ,autotrophic,unicellular,and eukaryotic.what kingdom does it most likely belong in

Answers

Answer:

This organism probably belongs to the Protist realm.

Explanation:

The Protist Kingdom is the group formed by living beings that may possess cell walls, eukaryotes, autotrophs, heterotrophs and unicellulars. This kingdom includes algae, protozoa and myxomycetes, which are living beings similar to fungi.

Although some species of these organisms cause various diseases, others play an important role in nature, such as algae. They release into the atmosphere dimethylsulfide gas (DMS), which is responsible for cloud formation and much of the planet's oxygen production, after all, 71% of its surface is covered by water and in these environments normally found. seaweed They are still the basis of the aquatic food chain.

As the temperature of sea water decreases, its density

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Hello there, and thank you for asking your question here on brainly.

Answer: The density increases, because the deeper you go, the colder in gets, and the colder you get, the more dense it feels. When you're higher in the sea, the temperature is higher, which means the water is less dense.

Hope this helped you! ♥
The further you go down into any body of water, the colder the water gets, so, the further you go down the more the density increases. Density can also be related to pressure, which is why the further down you, the more weight is on your chest, even the most advanced subs can't reach the bottom of parts of the ocean or else they will be crushed like tin can. 

Elaine feels that her life is empty. she has lost interest in career and hobbies, and she wonders if she would be better off dead. she is most likely suffering from a(n):

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A mood disorder

Mood disorders are illness in which an individual experiences serious change in mood or emotions. An individual with this disorder may feel sad or empty. Depression is the common feature of mood disorder. Major depressive disorder, bipolar disorder, persistent depressive disorder and seasonal affective disorder are all illnesses under mood disorders. For the question given above, Elaine is most likely suffering from a mood disorder.






Hair and nails are apart of the ________ system

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The Integumentary System. I might be wrong but I just looked it up and that's the answer I got. Hope it helps:)

integumentary system!

__________ is the breeding of fish in freshwater ponds, lakes, and canals or in fenced-off coastal bays and estuaries or enclosures.

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Aquaculture is the breeding of fish in freshwater ponds, lakes, and canals or in fenced-off coastal bays and estuaries or enclosures. Aquaculture serves many purposes which include food production for human consumption, wild stock enhancement, fish culture for zoos and habitat restoration.






Reductions in the population size of the graceful antelope, a hypothetical species, has led to inbreeding. deleterious after-effects of that inbreeding are leading to further decreases in the population size of this species. this pattern is an example of a(n)

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The answer is an extinction vortex. This occurs when a population is too small to recover its population hence plummets into extinction.  This is because the population does not have a diverse genotypes on which natural selection can act on to enable the population to adapt. Additionally, the chances of homozygosity for recessive alleles is high hence decreasing the population even further.

vertebrates and tunicates share _____.

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A vertebrate is an animal that has a spinal cord which is surrounded by bone/cartilage just like us humans while tunicates are marine animals. They both share hollow nerve cords, dorsal (which is the back of an animal/human), and notochord (a skeletal rod surrounded by cartilage). 

I hope I was able to help you. :)

Vertebrates and tunicates share a common chordate ancestry

Vertebrates and tunicates share a common ancestry and are both members of the phylum Chordata. Chordates are characterized by the presence of a notochord, a flexible rod-like structure that provides support.

During their embryonic development, both vertebrates and tunicates possess a notochord, which is one of the defining features of the phylum Chordata.

The notochord, typically present in the early stages of development in both groups, plays a crucial role in providing structural support.

In vertebrates, the notochord is eventually replaced by the vertebral column, while in tunicates, it regresses as they mature into their adult form.

Additionally, both vertebrates and tunicates exhibit a hollow, dorsal nerve cord, another distinctive characteristic of chordates.

This nerve cord develops into the central nervous system, which is the brain and spinal cord in vertebrates, and a simplified nerve ganglion in tunicates.

Despite these similarities, it's important to note that tunicates and vertebrates have significantly diverged in terms of their adult morphology and lifestyle.

Vertebrates are complex, multicellular organisms with well-developed nervous, circulatory, respiratory, and skeletal systems.

In contrast, tunicates, also known as sea squirts, are simpler, filter-feeding marine organisms.

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What percent of earths water is found in the ocean ?

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Hi there!

Earth is covered in about 71% water. 96.5% of the water on earth comes from our oceans. 

Hope this helps you!

~DL

A 9-year-old boy was electrocuted when he stuck a pin into an electrical socket. your initial assessment reveals that he is pulseless and apneic. a second ambulance is dispatched to the scene for backup. as your partner begins cpr, you should:

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let him die because he pulseless first of all and also has apeneic

The angiosperms are the sister phylum to which gymnosperm phylum?

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The flowering plants, also known as angiosperms, are similar to gymnosperms which are capable of producing seeds. However they are distinguished from the latter by characteristics which includes flowers, endosperms within the seeds and the production of fruits that contain the seeds. They are the sister phylum to the following four gymnosperm groups namely, the conifers, cycad, gingko and gnetophytes.

What is the name for the protein that forms the basic fibers of body tissue?

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I think it was called colagen, or something.

It appears that the same lifestyle factors that contribute to the development of _____ also increase the risk for vascular dementia.

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It appears that the same lifestyle factors that contribute to the development of Cerebrovascular disease also increase the risk for vascular dementia. Vascular dementia describes the problems with reasoning, planning, judgement, memory and other thought processes caused by brain damage from impaired blood flow in the brain. 

The neurotransmitter _____, which helps to control the preciseness of the signal sent from one neuron to another, decreases with aging

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The neurotransmitter serotonin, or 5-HT, decreases with aging and is essential for regulating functions like mood and memory. Declines in serotonin levels affect neurotransmission precision in various brain areas, which could explain associated impairments in cognitive and motor functions observed in the elderly.

The neurotransmitter in question is serotonin, which is known scientifically as 5-hydroxytryptamine (5-HT). Acting both as an inhibitory neurotransmitter and a hormone, serotonin is crucial for regulating mood, behavior, sleep, and memory, functions that are known to decline with age. The levels of serotonin receptors and the serotonin transporter (5-HTT) are reported to decrease as one ages, particularly affecting the frontal cortex, thalamus, midbrain, putamen, and hippocampus. Besides serotonin, glutamate, the brain's primary excitatory neurotransmitter synthesized by neuronal and glial cells, is also implicated in neurodegenerative disorders and is found to decline in various brain regions with aging. Therefore, the decline in serotonin (and glutamate) is related to a less efficient neurotransmission, potentially impacting precision in neuronal communication.

Cleavage as part of embryonic development is distinctive because it involves ________.

Answers

Cleavage as part of embryonic development is idiosyncratic since it involves the cell division which includes the process of mitosis with minimal or no growth in successive divisions. Hope this is right and the correct answer you are looking for.
Answer: cell division by mitosis with little or no growth between successive divisions

Cell divisions in the embryonic development happen fast. This makes the cells don't have enough time to grow its membrane and its organelles. The lack of growth makes the cell size seems smaller as the division keep happening. 

The ________ functions in the control of movements of the upper legs and the trunk. corticobulbar pathway rubrospinal tract lateral corticospinal tract ventral corticospinal tract spinothalamic tract

Answers

The right option is lateral corticospinal tract

The lateral corticospinal tract functions in the control of distal muscles such as those of the limbs and the trunk. About 90% of the fibers that travel in the corticospinal tract are found in the lateral corticospinal tract which makes it the main component of the corticospinal tract.






Final answer:

The ventral corticospinal tract is tasked with controlling movements of the trunk and upper legs. It coordinates both sides of the body for movement and posture, particularly influencing the axial muscles of the trunk.

Explanation:

The ventral corticospinal tract functions in the control of movements of the upper legs and the trunk. This tract is part of the corticospinal tract, which is a major descending pathway responsible for the conscious or voluntary movements of skeletal muscles. Movements of the body trunk are coordinated by the anterior corticospinal tract, which affects both axial and appendicular (limb) muscles but is particularly important for postural muscles that control broad movements of the body. The ventral corticospinal tract does not decussate at the level of the medulla and thus primarily influences movements on the same side of the body. These axons remain anterior as they travel through the brain stem and spinal cord, synapsing with lower motor neurons in the medial regions of the ventral horn, which control the axial muscles of the trunk.

As part of a simulation of evolutionary process, a teacher drops 500 red and 500 green jelly beans on green grass. the red and green jelly beans represent individuals of the same species. students represent predators and pick up as many jelly beans as possible in 30 seconds. which prediction is most accurate?

Answers

The answer should be: green individuals are more likely to survive and reproduce

In a green grass field, green beans will be hard to be noticed. Unlike the green beans, the red beans will have a high contrast to the green color of the grass. This makes the red bean easier to found and eaten by the predator. Green individuals will be more likely to survive and reproduce because it could blend with the grass.

Final answer:

In a simulated evolutionary process using jelly beans as prey and students as predators, it would be predicted that more red jelly beans would be picked up than green ones due to the green jelly beans' camouflage in a green grass environment, illustrating natural selection.

Explanation:

In the jelly bean simulation, the most accurate prediction would involve predation pressure and camouflage. As the simulation takes place, students will likely pick up more red jelly beans than green jelly beans because the red ones will stand out against the green grass, and the green ones will be harder for the 'predators' students to see. This represents a selection pressure similar to that in nature, where individuals that blend into their environment are less likely to be preyed upon, and therefore, more likely to survive and reproduce.

If the red jelly beans are deemed to be eaten and thus removed from the gene pool, we would expect the population of jelly beans over subsequent generations to have more green individuals. This simulation represents natural selection, where the green jelly beans have a survival advantage in this environment due to their camouflage, a form of natural selection that can lead to evolutionary changes in the population over time.

________ refers to a genetically influenced weight range for an individual that is maintained by biological mechanisms that regulate food intake, fat reserves, and metabolism.

Answers

Set point is the genetically influenced can individual weight range for an individual; it is maintained by biological mechanisms that regulate food intake, fat reserves, and metabolism. This is the weight range in which the body is programmed to function optimally. According to the set point theory one's body will fight to maintain that weight range.

The __________ used a buffer overflow exploit in "fingerd" as one of its attack mechanisms.

Answers

The Morris internet Worm used a buffer overflow to exploit in "fingerd" as one of its attack mechanisms. A buffer overrun, overflow, or overwrite is a condition at an interface under which more input can be placed into a buffer or data holding area than the capacity allocated, overwriting other information. The buffer overflow type of attack has been known since it was widely used by Morris Internet Worm in 1988.

A client severely injured in a motor vehicle accident is rushed to the health care facility with severe head injuries and profuse loss of blood. which sign indicates approaching death?

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profuse loss of blood. if an individual loses a certain amount of blood they can die (bleed to death). head injuries tend to bleed a lot; sometimes blunt force trauma to the head can result in internal hemorrhaging and swelling of the brain. In this case not knowing the condition of his brain with the information given the profuse loss of blood is the most likely answer.

Final answer:

The correct option is Client's breathing becomes noisy. Approaching death in a severely injured client is indicated by noisy breathing, known as a 'death rattle.' Other signs include a weak or absent pulse, cool extremities, pale skin, and decreased urine output.

Explanation:

Approaching death is indicated by several physiological changes as the body begins to shut down. In the case of a client severely injured in a motor vehicle accident with severe head injuries and profuse loss of blood, the sign that indicates approaching death would be noisy breathing, often referred to as a "death rattle," which is due to relaxed throat muscles.

In addition, other signs that death is imminent include a weak or absent pulse, extremities that are cool or cold to the touch, skin that becomes pale and waxen, and a noticeable decrease in urine output.

Healthcare providers must recognize these symptoms to provide the appropriate end-of-life care and prepare the client's loved ones for the impending loss.

The first cell type likely to respond to invasion by a pathogenic microbe is

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Hello, and good afternoon. Great question. Moreover, the only immunization cell type responders are the white blood cells that usually release pathogenic eradicators called antibodies.

​amenorrhea is a condition in which a female experiences excessive flow during menstruation.

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The answer would be false



"do b-cells ever interact with pathogens that have infected a cell"

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I think they don't; they only interact with free-floating pathogens. B-cells directly bind to free-floating pathogens. B-cells function in the humoral immunity component of the adaptive immune system by secreting antibodies. BCRs allow the B cells to bind to a specific antigen, against which it will initiate an antibody response. 

Studies suggest that the amount of caffeine in as many as ________ of coffee per day yields little health risk.

Answers

For the answer to the question above,
Studies suggest that the amount of caffeine in as many as "four cups" of coffee per day yields little health risk.
The answer is "4 cups".

Caffeine in the coffee makes our heart beat faster. Irregularities on the heartbeat will give you a heart disease in the future.

Final answer:

Given the potential risks, there is no established amount of caffeine that can be defined as 'safe' for all individuals. However, for most people, normal consumption of caffeine is seen as not harmful. Considerable over-consumption can lead to mental and physical health issues.

Explanation:

It's important to understand that caffeine, a psychoactive drug, is widely consumed around the world and is legal. Nonetheless, high consumption levels can cause health issues such as insomnia, agitation, irregular heartbeat, and muscle twitching. As an example, Kromann and Nielson reported on a case in 2012 in which a woman who was consuming three liters of soda each day experienced both physical and mental health issues. It wasn't until she reduced her intake to less than 12 ounces a day that her health improved.

Studies have yet to establish the exact 'safe' quantity of caffeine, however, normal consumption is generally considered to be non-harmful. Addiction can occur in those who routinely consume substances with caffeine, leading to physical and mental health struggles. Caffeine consumption is particularly cautioned against in pregnant individuals, although no negative effects have been definitively proven.

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Which vitamins and minerals promote healthy bones?

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Hello!

Calcium or vitamin C is good for the bones.

hope this helps!

~Lex

Certain green leafy vegetables have a very low calcium bioavailability due to the presence of low calcium content. low vitamin d content. naturally occurring binders. high magnesium content, which lowers calcium absorption.

Answers

The answer would be: naturally occurring binders.

Some leafy vegetables like spinach have high in calcium. But spinach has a substance that binds calcium called oxalic acid. This substance will prevent the absorption of the calcium, makes it wasted. There are other calcium binders that can be found like phytate or magnesium.
Final answer:

Calcium bioavailability in green leafy vegetables can be influenced by vitamin D and other nutrients. A deficiency in vitamin D can compromise calcium absorption, while nutrients like Vitamin K support bone mineralization and regulate bone growth in combination with vitamin D.

Explanation:

The presence of certain elements in green leafy vegetables can influence calcium bioavailability, affecting calcium absorption in the body. An important nutrient like calcium is found in milk, dairy foods, green leafy vegetables, and other foods like nuts, beans, seeds, and shellfish. However, the absorption of calcium in the intestines is dependent on activated vitamin D. A deficiency in vitamin D can result in decreased calcium absorption, leading to potential health issues like rickets in children, osteomalacia in adults, and contributing to osteoporosis. Other nutrients like Vitamin K, which is also found in green leafy vegetables, support bone mineralization and work in synergy with vitamin D in regulating bone growth.

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which urinary structure serves as the transporter for urine toward the bladder?

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The answer is ureter. They concentrate the urea from nephrons and collect in the bladder.  They are larger in diameter (25–30 cm) compared to the proximal and distal tubules, loop of henle and collecting tubules. There is one ureter for each kidney connecting to the bladder. The ureter has smooth muscles that cotract and direct the urine to the bladder


Final answer:

The ureters are the urinary structure that transport urine from the kidneys towards the bladder. They use a process called peristalsis to move the urine in one direction.

Explanation:

The urinary structure that serves as the transporter for urine toward the bladder is known as the ureters. The ureters are a pair of muscular tubes that propel urine from the kidneys to the urinary bladder. The urine is produced in the kidneys, and from there it goes down the ureters into the bladder. The bladder then stores this urine until it is released from the body through another structure called the urethra.

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What is the advantage of the gram stain over the simple stain?

Answers

Gram staining identifies different bacteria types and it separates bacteria into two groups (gram negative and gram positive) based on their cell wall constituents through the use of different special dyes. Simple staining involves the use of a single stain solution and is unable to identify the exact organism.






Both Earth and the planet Venus have a large amount of water vapor in the atmosphere, and have extensive volcanic activity throughout history. Does this observation support or disprove the inside-out model? Supports, because Venus is near Earth and would have been hit by all the same comets. Disproves, because a single observation can discredit a theory. Disproves, because data taken on Venus is not applicable to Earth. Supports, because it shows that volcanic activity may be related to water vapor.

Answers

i would say it supports the inside out model. because both planets are similar

Supports, because it shows that volcanic activity may be related to water vapor.

Almost no species ever reaches its biotic potential—the population size that the species could produce if all individuals survived and produced offspring. anything that prevents the species from reaching its biotic potential is called a

Answers

Out of the following given choices;

A.    symbiote.                                                                   C.  capacity limiter

B.      producer.                                                                  D.  limiting factor

The answer is D. Limiting factors limit the ideal growth rate of a population. Examples of these limiting factors include space (low space) and food (scarce food). These limiting factors would limit the population growth rate either by causing emigration, increases birth rate, and decrease birth rate.

Transcription makes which nucleic acid?
Protein
RNA
DNA
Polypeptide

Answers

The answer is RNA. RNA is composed of ribose sugars monomers. Their difference with deoxyribose sugars is that there have an H other than an OH in the 2’ carbon of their rings. In addition, RNA has similar bases as DNA other than Adenine which is replaced by Uracil in RNA.






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Match the description to the word.1.a line of verse consisting of five feet1pentameter2.a foot having one accented syllable followed by one unaccented syllablesonnet3.a poem having fourteen lines and a formal arrangement of rhymes, usually written in iambic pentameterquatrain4.a poem that tells a storytrochee5.a stanza or poem with four lines, usually with alternate rhymesballad What was the effect of the Platt Amendment?(A) It allowed Cuba to sign treaties with other countries without U.S. approval.(B) It gave the United States the right to preserve order as needed in Cuba.(C) It established a civil government in Cuba.(D) It expanded the rights of Cubans. The two square pyramids are similar. Find the total volume of both pyramids if the ratio of their surface areas is . 9/16 15 POINTSWhen calcium forms an ion, what is its charge? 2+ 3+ 2- 3- Choose the scientific notation that best represents the standard notation given. 0.000000000143 g What are you required to have in order to receive financial aid for college? The patient is here because the cyst in her chest has come to a head and is still painful even though she has been on antibiotics for a week. I offered to drain it for her. After obtaining consent, we infiltrated the area with 1 cc of 1% lidocaine with epinephrine, prepped the area with Betadine and incised and opened the cyst in the relaxed skin tension lines of her chest, and removed the cystic material. There was no obvious purulence. We are going to have her clean this with a Q-tip. We will let it heal on its own and eventually excise it. I will have her come back a week from Tuesday to reschedule surgery. What CPT and ICD-10-CM codes are reported? Which recovery method enables you to retain your personal data, windows store apps, and basic settings while resetting windows back to a good working state? The 2000 presidential election was determined by the contested results of When you send a formal report, proposal, brochure, or other similar material, you should include a short message, often called a ______________ , iden? When climbing a mountain, we can observe transitions in biological communities that are analogous to the changes ________? Cindy's exercise routine consists of lifting weights every other day. While she can bench press more than her body weight, she has trouble keeping up with her friend Sandra on bike rides. In a couple of sentences, explain what Cindy can do in her training to increase her muscular endurance. Can someone help me answer this please What is one purpose of genocide?A) to create a politically stable governmentB) to persecute a minorityC)to eliminate equal distribution of power in a stateD) to end a violent civil war How English has evolved since the beginning? Tyler returned home after college to live with his parents. this was to be a temporary arrangement; however, days became months and months became three years. tyler's dad wonders if he will ever move out and get his own apartment. tyler is part of an increasing trend. one-fifth of all 25+ year olds live with their parents. demographic researchers refer to this group that tyler is in as which statement best describes how a muscle moves a bone WHAT IS THE MAIN IDEA OF THIS TEXT!!!!!!!!!!! IN COMPLETE SENTENCES PLEASEWith renewed hope that there was some truth to the legends, I looked for new research. I found that some experts disagreed with popular arguments against dragons. They suggested that a dragon could have four stomachs like a cow. If it created stomach gases like birds, it might create enough to lift itself off the ground. This would give it the ability to fly. If it forced out air when diving toward the earth, it might release gases which could ignite into flame. When the animal died, the stomachs would release strong acids that would dissolve its dead body over time. Biologists backed up these ideas with sketches and models based on known animals. Not everyone agreed with these ideas, but many of the things we accept about dinosaurs and other extinct species started the same way. Political speeches are usually _____ speeches.impromptupersuasiveentertaininginformative Select all that apply. Select the items that apply to money made from precious metals and paper. easily divisible valuable durable hard to transport You have 23 coins, including nickels, dimes, and quarters. if you have two more dimes than quarters, and the total value of the coins is $2.50. how many of each kind of coin do you have?