In genetic engineering, the staggered cuts in dna that allow genes to be spliced together are made by __________.

Answers

Answer 1

The answer is restriction enzymes. These staggered ends are important in recombination of DNA since they allow DNA strands with complementary sticky ends to be easily joined into one piece by DNA ligase. Other restriction enzymes produce blunt ends. These are harder to join by DNA ligase. An example of a restriction ezyme if EcoRI







Related Questions

Which statement best describes an effect of the low density of frozen water in a lake?

A. When water freezes, it contracts, decreasing the water level in a lake.
B.Water in a lake freezes from the bottom up, killing most aquatic organisms.
C.When water in a lake freezes, it floats, providing insulation for organisms below.
D.Water moves thermal energy from the land around a lake, causing the lake to freeze.

Answers

C. When water in a lake freezes, it floats, providing insulation for organisms below.

This  best describes an effect of the low density of frozen water in a lake.

The statement which is describes an effect of the low density of frozen water in a lake when water in a lake freezes, it floats, providing insulation for organisms below. Thus, option C is correct.

What is density?

Density has been defined as mass per unit volume it means that mass is present in one meter cube.

S.I unit of density is kg/meter^3.

Mathematically,

Density = Mass/Volume.

Water has been defined as the water present under the ground and water below the soil as well as in the crack of the earth. The percentage of freshwater in the world could be 30%. There are mainly three types of groundwater and these are hand-dug wells, springs, and drilled boreholes.

The water stored under the ground are obtained from rain and stored in the ground so that it can be used later. Ground water has very clean and fit for drinking and the reason could be that the soil present on top and work as filter and the groundwater has free from all microorganisms.

Therefore, The statement which is describes an effect of the low density of frozen water in a lake when water in a lake freezes, it floats, providing insulation for organisms below. Thus, option C is correct.

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What producers serve as the basis of the food chain in this layer of the ocean answers?

Answers

In the first segment that covers the top part of the ocean the whale sharks producers serve as the basis of the food chain. In the top layer of the ocean enough sunlight hits the water which makes it a good place for planktons to grow. These planktons are then eaten by the whale sharks.

If blood is in short supply, which blood type would be the most beneficial to have on hand if someone needed a blood transfusion?

Answers

The best type of blood to store in this situation is 0-, as all people with other types of blood can receive transfusion of this blood. The worst is AB+ as only AB+ can receive this blood.

Hope this helps!

-Payshence xoxo

Answer:

0-

Explanation:

Pyruvate dehydrogenase is _______________ when the ratio of atp to adp (atp/adp) are high.

Answers

Pyruvate dehydrogenase is inactive when the ratio (ATP/ADP) are high. Phosphorylation of PDH is mediated by a special regulatory eznyme, pyruvate dehydrogenase kinase. This enzyme is part of the PDH multienzyme complex. Phosphorylation inactivates pyruvate dehydrogenase. The kinase is, in turn, subject to allosteric activation by NADH and acetyl-CoA, while it is inhibited by ADP, NAD+ and by free coenzyme A. When the ratio of ATP/ADP increases, pyruvate dehydrogenase kinase is activated which then inactivates PDH.

Answer:

Inactive

Explanation:

Pyruvate dehydrogenase controls the production of acetyl CoA.  This conversion is regulated by regulators like ADP, ATP, AMP and NADH. While ADP and AMP are regulators that enhances the activity of the Pyruvate dehydrogenase enzyme, regulators like ATP and NADH reduces the activity of the Pyruvate dehydrogenase enzyme. Thus, when the ratio of ATP to ADP is high then the inhibitory regulator i.e ATP is in large amount than the ADP. Hence, ATP reduces the activity of enzyme Pyruvate dehydrogenase and makes it inactive.

This diagram shows that embryos of certain species develop almost identically in the earliest stages of embryonic development. These physical similarities indicate that there are also genetic similarities among these organisms. The physical and genetic embryological similarities among these organisms suggest that they A. descended from a common ancestor. B. have multiple vestigial organs in common. C. are members of the same genus. D. hunt and breed in similar habitats.

Answers

The correct option is A.
The observation that the embryos of certain species have physical and genetical similarities in the earliest stages of embryonic development shows that individual development of an organism shows its revolutionary history and this is taken as an evidence of common ancestry. Scientists takes this observation to mean that all life on earth developed from a single ancestor.

Earth’s crust continuously melts and solidifies in the upper mantle. Why does oceanic crust solidify faster than continental crust?

A Continental crust spans a wider area and is generally thinner than oceanic crust.
B Magma beneath oceanic crust is a lower temperature than magma beneath continental crust.
C When magma comes into contact with ocean water it cools faster than when it reaches continental rock.
D Salt in ocean water accelerates the solidification process by way of chemical reaction.

Answers

The correct answer here is C. Water molecules are more free to move, so they will be able to engage in rapid and fluid contact with magma. This is in contrast to fixed continental rock, which may have quick initial heat transfer but which tapers off quickly because the rock is not flexible enough to send cooler rock towards the magma.

C When magma comes into contact with ocean water it cools faster than when it reaches continental rock.

The end of the Paleozoic Era was signaled by

Answers

mass extinction of land and water animals

Paleozoic era lasted for millions of years ago. It is divided in 6 different periods. It also signal various events in evolution which include the development of most invertebrate groups. Fish and fish like vertebrates arose from the early Paleoozoic and comprise more than 50 percent of the diversity of vertebrates that inhabits today. This period ended when the mass extinction of animals occured.

what is a component of a phospholipid

Answers

polar head group, hydrophobic tail.

Hope this helps!

-Payshence xoxo

Primary consumers depend on energy from plants for survival. Higher consumers depend on energy from primary consumers for survival. Which of the following is also true about plants and consumers?

Answers

The answer is C
The more plants there are, the more consumers there are. If there aren't enough plants, the consumers have no way of getting energy, and therefore cannot survive. The species also matters, because certain species of plants can only be eaten by certain species of consumers.

Final answer:

The true statement about plants and consumers is that primary producers convert light energy through photosynthesis and consumers gain energy by feeding on them or on other consumers.

Explanation:

Primary consumers depend on energy from plants for survival, while higher consumers depend on energy from primary consumers. The statement that is also true about plants and consumers is that primary producers convert light energy by photosynthesis and consumers gain energy by feeding on them or other consumers which have consumed these producers.

Within ecosystems, the base of the food chain is typically occupied by photosynthetic organisms, referred to as producers. These producers, like plants or phytoplankton, harness solar energy and convert it into chemical energy through photosynthesis. Primary consumers, such as herbivores, directly consume these producers and gain their energy from them. Subsequent consumers, including secondary and tertiary consumers, obtain their energy by consuming organisms from the previous trophic levels.

It's important to clarify that primary producers do not convert light energy through chemosynthesis, but rather, through photosynthesis. While chemosynthesis is another form of biological energy conversion, it is not relevant to the typical processes that plants use to acquire energy.

Which list places the layers of the sun in the correct order from innermost to outermost?

A. Corona, photosphere, chromosphere

B. Photosphere, convective zone, radiative zone

C. Convective zone, chromosphere, corona

D. Radiative zone, corona, convective zone

Answers

The correct answer is: C. Convective zone, chromosphere, corona.

The Sun can be divided into six layers (from innermost to outermost):

1. Core-the interior of the Sun which produces heat and light

2. Radiative zone-high density; radiation

3. Convective zone

4. Photosphere – visible layer

5. Chromosphere

6. Corona


Answer:

Convective, chromosphere, corona best fits the order in which the sun is layered

Explanation:

How many teeth are found in pan troglodytes maxilla?

Answers

The answer is 16.

Pan troglodytes
is the scientific name for the common chimpanzee, or commonly referred to as just chimpanzee, from the order of Primates. Regarding their dental development, we have much more information about the chimpanzee that about other great apes still in existence. This is a species that shares the most genetic information with humans.

The chimpanzee has 32 teeth (just like humans), which are pretty similar to human teeth. We have the same dentition pattern (same number in the same order). They use them not just to break down plants, but they also have large canines that allow them to bite into flesh. The maxilla is the upper jawbone that animals have which is formed when two maxillary bones are fused.

To answer your question, the jaw of the chimpanzee has 32 teeth, 16 in the maxilla, and 16 in the mandible.

The Mohs scale is used to determine a mineral's

Answers

it's hardness
Example 10 is diamond
and 1 is Talc

What is the estivation, how is it similar to hibernation

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estivation is prolonged torpor or democracy of an animal during a hot or dry period. Hibernation and estivation are similar in that they are adaptations to harsh.

Which part of the brain serves to regulate the endocrine system ?
Adrenal gland
Thymus
Hypothalamus
Thyroid

Answers

Answer:

The answer would be hypothalamus. Hypothalamus is a part of the brain in which regulates the endocrine system.

plz help me out and say thanks ;-;

During the process of biological _______ , the concentration of pollutants, such as DDT or mercury, is multiplied as it passes up a food chain .
A) diversification
B) invasion
C) magnification
D) multiplication

Answers

Biological magnification

The increase in concentration of a substance such as toxic chemical in the tissue of an organism at successively higher level in food chain can be refers to as Biological magnification or Biomagnification. It also a process where certain substances such as heavy metals or pesticides find their way into the rivers, lakes or ocean and then move up the food chain in greater concentration as they are incorporated into diet of aquatic organisms.


Carl Woese introduced the concept of domains in the 20th century. Which characteristic of scientific knowledge does this highlight?
It can be modified.
It is based on predictions.
It has only a single interpretation.
It will remain the same over years.

Answers

Answer:

i believe it is a  

Explanation:

The scientific knowledge given by Carl Woese is highlighted here in that it can be modified. therefore, option "A" is correct.

What is the three-domain system given by Car Woese?

Carl Woese introduced the three-domain system in 1990. He divided the organisms into three domains: Archaea, Bacteria, and Eukarya. prokaryotes were divided into two domains therefore Archaea and Bacteria.

The Archaea domain consists of organisms that are the oldest living and can live in extreme environmental conditions. In the domain, bacteria consist of organisms that lack membrane-bound organelles whereas in the domain eukarya consist of protista, fungi, plants, and animals.

Therefore, Carl Woese's three-domain system includes Archaea, bacteria, and eukarya.

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Help #13 I can’t figure this one out

Answers

Hello,

The answer is option D "reduce the high levels of carbon dioxide emitted into the air".

Reason:

Making nuclear energy requires a lot of carbon dioxide therefore its all going into the air causing the Earth to heat up then causing Global warming and even maybe climate change so scientists have been trying to come up with ways they can make nuclear energy but reduce the amount of carbon dioxide it needs to be made.

If you need anymore help feel free to ask me!

Hope this helps!

~Nonportrit

A woman develops skin cancer after prolonged exposure to ultraviolet radiation from sun tanning. Would her future children be at risk of inheriting this skin cancer?



Yes, if she had a baby after developing skin cancer.

Yes, this is a mutation in the DNA of her somatic cells.

No, this condition does not affect the women's gamete cells.

No, babies cannot get skin cancer because they are immune.

Answers

The answer is b I know so

Answer: B is the correct option. Yes, this is a mutation in her DNA of her somatic cells.

When the woman develops cancer due to prolonged UV radiation, the cancer cells spread and eventually all the somatic cells which are non-reproductive egg or sperm cell also carry the DNA of the cancer cells. So the future children will have these mutations in their cells. Mutation in the BRAF gene is the main cause of several types of melanomas. The other gene that is commonly mutated is p53 which is a tumour suppressor gene in normal conditions and on mutation it causes cancer causing the tumours.


"which part of the cell would be of most interest when in studying production of atp and nadph?"

Answers

mitochondria bc that where cellular respiration occurs

What is the most likely cause of this person's color blindness? what does 20/300 vision indicate, and how is that corrected by lasik surgery?

Answers

Final answer:

Color blindness is caused by genetic factors affecting the cones in the retina and includes three types related to different cones. Vision of 20/300 is severely impaired, and LASIK surgery can correct this by reshaping the cornea. Presbyopia affects reading vision due to lens flexibility issues and may remain after LASIK surgery.

Explanation:

Color blindness is typically caused by genetic factors that affect the cones in the retina of the eye. The three types of color blindness are: protanopia (red), deuteranopia (green), and tritanopia (blue). Each type corresponds to the deficiency or absence of different cone types in the retina. In context of genetics, the Law of Dominance explains how a dominant allele can mask the presence of a recessive allele, which may relate to inheritance patterns in color blindness.

Regarding visual acuity, a measure of 20/300 indicates that what a person with normal vision can see at 300 feet, someone with a 20/300 vision sees at 20 feet, reflecting severe visual impairment. LASIK surgery corrects this by reshaping the cornea to correct the focus of light onto the retina.

For individuals with presbyopia, LASIK can correct distant vision but will not solve the issue of close-up focus required for reading because presbyopia is related to the eye's lens flexibility, which diminishes with age. The patient may still need reading glasses after LASIK. The procedure of LASIK involves a fixed percent uncertainty in the reshaping of the cornea, and those requiring greater correction have a higher risk of not achieving normal distant vision due to the greater variability in the surgical outcome.

completely factor 4s^4 +8s^3-60s^2

Answers

factoring the expression: 4s^2(s-3)(s+5)

Subtilisin is used in some laundry detergents to help remove protein-type stains. what unusual kind of stability does this suggest for subtilisin?

Answers

Out of the following given choices;

a) The enzyme must be stable to very high pressures.

 

b) The enzyme must be stable in the presence of hydrogen.

 

c) The enzyme must be stable both in the presence of detergents and to high pressures.

 

d) The enzyme must be stable both in the presence of detergents and to moderately high temperatures.

 

The answer is D. Subtilisin is a non-specific protease enzyme hence its use to remove protein stains. For its to be used with detergents it has to be stable in the presence of detergents. Since detergents work optimally in moderately high temperatures, Subtilisin must also be stable at the same temperatures.


Plastics are artificial substances. However, the materials needed to produce plastics are often crude oil. Which is a reasonable conclusion based on this fact?

A. Plastics require oil and therefore will be lighter than any other material

B. Plastics require oil and therefore must be more costly to use than other material

C. Plastics require oil and therefore are renewable resources

D. Plastics require oil and therefore are nonrenewable resource

Answers

Plastics require oil and therefore are nonrenewable resources

Final answer:

Plastics are made from petroleum-based products, which are finite resources being depleted, therefore, they are classified as nonrenewable resources. Therefore, the correct answer is D.

Explanation:

Plastics are synthetic materials commonly derived from petrochemicals such as crude oil. Because plastics are made from oil, which is a finite resource with reserves being depleted, a reasonable conclusion based on this information is:

D. Plastics require oil and therefore are nonrenewable resources.

The production of plastics does indeed lead to an increase in carbon emissions and contributes to global warming, as it requires a significant amount of energy and resources. The immense scale of plastic use and production has led to an increase in environmental concerns, including waste management, pollution, and depletion of natural reserves. While plastics may replace heavier materials like metal and glass, their environmentally friendly status is challenged by their impact throughout their lifecycle and their non-biodegradable nature, leading to large amounts of persistent waste.

What do volcanoes have to do with the carbon cycle ?

Answers

The slow cycle returns carbon to the atmosphere through volcanoes. ... If carbon dioxide rises in the atmosphere because of an increase in volcanic activity, for example, temperatures rise, leading to more rain, which dissolves more rock, creating more ions that will eventually deposit more carbon on the ocean floor.

What species of bacteria or archaea might you find associated with these green beans?

Answers

Rhizobium affects the bean plants by providing them withnitrogen-fixed nodules that help them grow and get the nu-trients they need

Meiosis is the process that results in the production of the haploid number of chromosomes.
a) true
b) false

Answers

This is 100% true true true!!

Meiosis is the process that results in the production of haploid gametes, making the statement (a) true. It involves two rounds of division, producing four unique haploid cells that are essential for sexual reproduction.

Meiosis is indeed the process that results in the production of the haploid number of chromosomes, so the correct answer is (a) true. During meiosis, a diploid cell undergoes two rounds of nuclear division to produce four genetically unique haploid gametes. The sexual reproduction cycle begins with this essential step, as gametes are necessary for the fertilization process. In the first round of division, called meiosis I, homologous chromosomes separate, and in the second round, called meiosis II, the division of sister chromatids occurs, akin to what happen in mitosis. This process ensures the resulting gametes have half the number of chromosomes as the original cell, which is a critical aspect for maintaining the species-specific chromosome number during sexual reproduction.

Which of these is not an area that is prone to landslide hazards?

Answers

Thick, moist soils is not an area that is prone to landslide hazards.

A student wrote the following statements about ocean waves.

Statement 1: They have long wavelength.
Statement 2: They are driven by strong winds.
Statement 3: They originate as high amplitude waves.
Statement 4: They travel at very high speed.
Which of these statements best describe the properties of tsunami waves? (2 points)

Statement 1 and Statement 2
Statement 3 and Statement 4
Statement 1 and Statement 4
Statement 2 and Statement 3

Answers

Statement 2&3 maybe 4
I believe the answer to this is statement 1 and 4. 

The wavelength can range from 10km to 500km and the speed is 500 miles per hour.

hat does this suggest? What does this suggest? The presence or absence of four limbs is not a useful character for studying evolutionary relationships among vertebrates. Of the species in the table, rattlesnakes are most closely related to lampreys. Rattlesnakes are more closely related to bass than they are to frogs, turtles, and leopards. The presence or absence of four limbs, on its own, could be misleading when considering the evolutionary relationships of rattlesnakes.

Answers

The answer to this question is the presence or absence of four limbs, on its own, could be misleading when considering the evolutionary relationships of rattlesnakes.
This happens when we consider the phylogenetic relationships between different groups of organisms using just one trait or a gene can give misleading results. In this situation, the absence of limbs is a new characteristic (snakes used to have four limbs like all the other vertebrates, and phytons and boa snakes still have some skeletal elements of the limbs in their bodies) so if we used the absence of limbs as a defining trait, that would mean that the rattlesnakes are more related to lamprey which are also limbless, than to other reptiles that have limbs, which is incorrect. 

Match the blood type with the possible genotype(s) type a blood

Answers

Type A - AA or AO 
Type B - BB or BO 
Type AB - AB 
Type O - OO 
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How many questions are on the science aspire testA: 40B: 75C: 60D: 20 what are the mean median mode and range of the data set given the altitude of lakes in feet -9, -36, -23, -8, -17, -52, -27, and -36 A. mean=-25; median=-26; mode=-44; range=36 B. mean=-25; median=-36; mode=-36; range=44 C. mean=-26; median=-25; mode=-36; range=44 D. mean=-26; median=-44; mode=-25; range=36 . The latent heat in a substance has a negative value. Which of the following situations could be true?(Points : 1) A liquid is being heated to the boiling point. A liquid is changing to a gas at the boiling point. A liquid is cooling to the freezing point. A liquid is solidifying at the freezing point.****my answer is D is that correct?, What is the sum of 4.2 105 and 5.3 10^5? A.)95 10^5 B.)95 10^10 C.)9.5 10^5 D.)9.5 10^10 which of the following careers is part of the Printing Technology pathway?ComposerProofreaderTextile designerLithographer "The Perils of Indifference" begins with which memory of Wiesel's youth? A.Wiesel's meeting with the soldiers who helped him B.Wiesel's train ride to the concentration camp C.The last day before Wiesel was taken to a Jewish ghetto D.Wiesel's release from the concentration camp, What tactics does a financial planner apply to deal with a difficult client Who helped shape how judaism was practiced for the next several centuries? which of the following 20th-century figures influenced modernist literature through his groundbreaking work on subconscious? A. Albert Einstein B. Carl Jung C. Karl Marx D. Vladimir Lenin What are 5 things that a person with hoarding disorder would want me to know? On a circular playground, the distance from its center to the edge of the playground is 35 feet. What is the approximate circumference of the playground? what does it mean to "square a circle"? If a number of two words or less opens a sentence, it should be A. written in numerical form. B. removed from the sentence altogether. C. written out in text. D. moved somewhere else in the sentence. To do this, the scientist sedates and tags all of the lions within a pride. then, he places several remotely-controlled video cameras near the lions' den and performs an observational field study. he collects continuous video footage over the span of one year, analyzes the video, and then forms conclusions based on his observations. this example shows that A unilateral contract is formed at the moment when the contract is performed. Bacteria are excellent candidates for genetic engineering because? What are the four core values of deaf culture?a. self-determination, literature, internet access and family timeb. self-destruction, spirituality, literature, and internet accessc. self-actualization, healthy identity formation, internet access and family timed. self-actualization, spirituality, full access to communication and deaf schoolse. self-determination, full access to communication, healthy identity formation and information sharing? Read these lines from the poem. Alone the glory and the spoil I always laughing bore away; The triumphs, without pain or toil, Without the hell, the heavn of joy.What tone does the imagery in these lines create? Daniel has started a small shoe manufacturing company. he has a tight budget, and does not want the best equipment at the moment, so he is using equipment that is good enough to work with. in this scenario, daniel is _____. PLEASE HELP ME I WILL GIVE YOU ALOT OF POINTS!!!!!!!!!!!!!!!Scientists discovered that the inner core of Earth is a solid. Explain what evidence supports this thinking. Be sure to include how pressure, temperature, and density play a role.