Positive control: Known sample to validate gel and electrophoresis conditions. Negative control: No DNA to check for contamination.
In gel electrophoresis, a positive control and a negative control are essential components used to validate the experimental procedure and interpret the results accurately.
A positive control typically consists of a known sample or DNA fragment with a confirmed size or characteristic that is expected to migrate to a specific position on the gel. This control helps verify that the gel and electrophoresis conditions are suitable for DNA separation and that the experimental setup is functioning correctly. If the positive control produces the expected result, it confirms that the experimental conditions are optimal for detecting DNA fragments of interest.
On the other hand, a negative control contains a sample or buffer lacking the target DNA or any DNA at all. Its purpose is to detect potential contamination or unintended DNA amplification. If the negative control does not yield any bands on the gel, it indicates that there was no contamination introduced during the experimental procedure. This ensures the reliability of the results by ruling out the possibility of false-positive signals caused by contamination.
In summary, the positive control confirms the functionality of the experimental setup and validates the expected migration of DNA fragments, while the negative control verifies the absence of contamination and helps prevent false-positive results. Together, these controls ensure the accuracy and reliability of the gel electrophoresis experiment.
Some members of david's family have an autosomal recessive disease. david does not have the disease; neither do his parents, nor his two brothers. his maternal grandfather has the disease, his paternal grandmother has the disease, and his sister has the disease. draw a pedigree chart to represent the geno- types of all grandparents, parents, and children. next to each person, write his or her possible genotype
Ultimately, pelvic inflammatory disease in women is made possible because of the less-than-complete connection between the __________ and the __________.
Which factor do oceanographers determine by measuring the conductivity of a sample of seawater?
The correct answer is salinity.
Oceanographers can determine the salinity of the body of water in many ways, however, the two most usual methods are the application of salinometer or hydrometer. A hydrometer set comprises a hydrometer, a cylinder, and a temperature-salinity-density graph. The temperature is then determined and both are compared with the readings on the TSD graph. This then provides them the salinity, as salinity, temperature, and density are associated variables.
The hydrometer determines the density of water as higher salt constituent means denser water that makes the hydrometer to float higher in the cylinder. The oceanographers can also utilize a salinometer to find the salinity by calculating electrical conductivity.
Final answer:
Oceanographers measure the salinity of seawater by assessing its conductivity. Modern CTD instruments can measure a range of parameters, including conductivity, temperature, depth, light, and water clarity. The Practical Salinity Scale of 1978 is based on conductivity measurements.
Explanation:
Oceanographers determine the salinity of seawater by measuring its conductivity. This is because salinity can dramatically affect the water's ability to conduct electrical current. Higher salinity results in a higher conductivity due to the greater presence of ions. To facilitate this measurement, oceanographers use a CTD instrument, which stands for Conductivity, Temperature, and Depth, the most commonly measured parameters in oceanography. The Practical Salinity Scale of 1978, which defines salinity based solely on conductivity, is universally accepted and has replaced the historical method that involved a relationship with chlorinity.
Advanced CTD instruments are also capable of measuring additional parameters such as light and turbidity, which are indicative of water clarity and other environmental aspects. Measurements of conductivity, which are converted to salinity, along with temperature and pressure measurements, are crucial in determining the density of seawater, which in turn influences oceanic currents and the overall marine environment, including the community composition of free-living oceanic microbes.
The same tom and jane's blood donations are sent to a processing center where the blood cells are separated from the plasma in each of the two samples. the separated cells and plasmas are then sent to a hospital. a blood researcher wishes to use tom's blood in an attempt to extract and identify the a antigen. should she attempt the extraction process on his blood cells or on his plasma?
The blood researcher should attempt the extraction process on Tom's blood cells because the A antigen is found on the surface of red blood cells.
Explanation:The blood researcher should attempt the extraction process on Tom's blood cells.
The A antigen is found on the surface of red blood cells, so it is necessary to extract and identify it from the cells themselves.
If the researcher attempted the extraction process on Tom's plasma, they would not be able to find the A antigen as it is not present in the liquid portion of the blood.
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The A antigen, which the researcher is looking to extract and identify, is located on the surface of Tom's red blood cells, not in the plasma. Hence, she should use the blood cells for the extraction process.
Explanation:The researcher should attempt the extraction process on Tom's blood cells. The reason for this is because the A antigen, which the researcher is interested in, is located on the surface of the red blood cells, not in the plasma. ABO blood type, which includes type A, is determined by the presence of certain antigens on the red blood cells.
For example, individuals with type A blood have A antigens on the surface of their red blood cells.
These antigens are crucial for determining blood compatibility during blood transfusions. Karl Landsteiner, an Austrian biologist and physician, was instrumental in the discovery of these blood groups in the early 1900s, significantly reducing the risks associated with blood transfusion procedures.
It's important to note that blood typing also involves checking for the presence of the Rh antigen, another important factor in determining blood compatibility.
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In what step of meiosis would recombinants form and why
Answer needed ASAP!!! A rock that forms from cooling lava is classified as an _____.
The raw material that is added to a system for processing is known as _______. A. positive feedback B. negative feedback C. the input D. the output
Which of these species is most vulnerable to extinction?
Why do plant cells not need centrioles for mitosis or meiosis?
The step, during protein synthesis, of assembling proteins by decoding the information in messenger rna is called
A large water wave called a ______ can be generated by underwater earthquakes.
The surgeon explains the risks to you: infection occurs in 3% of such operations, the repair fails in 16%, and both infection and failure occur together in 1%. what percent of these operations succeed and are free from infection?
Mutations in what class of genes have probably been responsible for many of the changes leading to the great diversity of life existing today? genes that regulate mitosis genes for aerobic respiration dna repair genes developmental genes genes for digestive enzymes
Which type of tissue is responsible for secreting digestive enzymes?
Exocrine glandular tissues, including the salivary glands, gastric glands, and glands within the small intestine lining, as well as columnar epithelial cells, are responsible for secreting digestive enzymes. The pancreas is especially vital in this function, with its exocrine cells producing enzymes for digestion in the small intestine.
The tissue responsible for secreting digestive enzymes is known as exocrine glandular tissue. These glands include the salivary glands which release enzymes in the mouth, the gastric glands in the stomach lining, and glands within the lining of the small intestine. Furthermore, the pancreas is a significant source of digestive enzymes, with its exocrine cells producing enzymes that are transported via ducts to the small intestine for digestion. Cells such as the columnar epithelial cells in the intestine also contribute to this process by secreting digestive enzymes that facilitate chemical digestion.
Epithelial cells that specialize in secretion, including the columnar epithelium, contain structures called secretory granules. These granules are formed by the Golgi apparatus and upon reaching the cell surface, they release their content, which includes precursor forms of digestive enzymes, through a process called exocytosis.
Essentially, it is the exocrine cells, some of which are located in the pancreas, that are responsible for synthesizing and secreting pancreatic digestive enzymes into the ducts leading to the intestine, significantly aiding the digestion process.
Which hormone is secreted into the bloodstream by fatty tissue to signal the hypothalamus that the body has had enough food, reducing appetite and increasing the feeling of being full? leptin lippotor peptic acid adrenalin?
Which statements are true for chloroplasts? select the three that apply?
Suggest ways that plants could be altered to improve the world's food supply. Hint: The first sentence should express the main idea. No Bad answers.
Genetically modified (GM) plants designed to use light more efficiently produced a 20% greater yield in a study that could have significant importance for global food supplies.
What are GMOs?
Genetically modified organisms (GMOs) are living organisms whose genetic material has been artificially driven in a laboratory through genetic engineering. This creates combinations of plant, animal, bacteria, and virus genes that do not occur in nature or through traditional crossbreeding methods.
Thus, Genetically modified (GM) improve the world's food supply.
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Prior to the invention of refrigeration, meat was often preserved by soaking it in brine (a solution of water with a high concentration of salt) to kill any microorganisms that might have tried to use the meat as their own food source. Which of the following BEST explains how a brine solution could be lethal to those microorganisms? Water would move into the cells of the microorganisms by osmosis causing it to swell and burst. The salt would move into the cells of the microorganisms by facilitated diffusion causing the membrane to become rigid and less permeable. The salt would be pulled from the cells of the microorganisms by active transport removing necessary nutrients and destroying the cells. Water would move out of the cells of the microorganisms by osmosis and the cytoplasm would become dehydrated, killing the cells.
Water would move out of the cells of the microorganisms by osmosis and the cytoplasm would become dehydrated, killing the cells.
What type of blood cell transports oxygen throughout the body? what type of blood cell transports oxygen throughout the body? osteoblast leukocyte platelet erythrocyte?
Red Blood Cells.................................................
Calculate the frequency of b cells specific for antigen a on day 36 if the antibody concentration on day 36 is about 8 × 103. (for practice doing calculations with proportions, try this exercise. for practice doing calculations with numbers written in scientific notation, try this exercise.)
PLEASE HELP!
Which of the following effects of acid deposition most affects human health?
A)Acid rain damages human skin.
B) Acids remove lime from the soil.
C) Acids remove nutrients from the soil and cause malnutrition in crops
D) Acidic soils liberate toxic metals.
Which scenario best represents geographic isolation
A greenish area around a bacterial colony growing on blood agar indicates _______________.
Parents with traditional gender schemas are especially likely to raise children who show signs of
Final answer:
Children of parents with traditional gender schemas often demonstrate gender stereotyping, shaped by observed roles and societal influences. This stereotyping is anchored in gender schema theory, where children's experiences influence their understanding of gender roles, with less stereotyping occurring in more inclusive environments.
Explanation:
Parents with traditional gender schemas are especially likely to raise children who show signs of gender stereotyping. Gender schema theory suggests that children actively categorize and learn behaviors, activities, and attributes into gender-specific schemas. These schemas are observations of gender-related information and roles demonstrated by parents and society, such as distinct chores or expectations for boys and girls.
Research indicates that when fathers tend to be firmer with expectations for gender conformity, particularly with sons, the children may adhere more rigidly to traditional gender roles. This can result in boys becoming especially sensitive to their father's disapproval of engaging in activities perceived as feminine. Similarly, societal influences, including race and ethnicity, can further mold these gender schemas, with African American families more likely to model an egalitarian role structure than Caucasian families.
Children whose experiences are less confined by traditional gender roles tend to have a broader understanding of what activities are gender appropriate, allowing them to engage less in gender stereotyping. Thus, allowing a child to explore different toys and roles, regardless of gender, can promote a more inclusive understanding of gender roles.
Three cells with generation times of 60 minutes are inoculated into a culture medium. how many cells are there after 5 hours? three cells with generation times of 60 minutes are inoculated into a culture medium. how many cells are there after 5 hours? 15 180 900 32 96
After 5 hours, with a generation time of 60 minutes, there would be 96 cells in the culture medium.
In bacterial growth, the number of cells can be calculated using the formula:
Final cell count = Initial cell count×2 [tex]^{(number of generations)}[/tex]
Given the generation time is 60 minutes, and 5 hours is equivalent to 300 minutes, there are 300 minutes / 60 minutes/generation =5 generations.
If initially, there were 3 cells, the number of cells after 5 hours would be:
Final cell count = 3×32 = 96 cells
Therefore, after 5 hours, there would be 96 cells in the culture medium if three cells with a generation time of 60 minutes were inoculated.
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After 5 hours, with an initial count of 3 cells and a generation time of 60 minutes, there will be 96 cells in the culture medium. This is calculated using the exponential growth formula. The cells double every hour, leading to a total of 5 generations. Option E is correct .
To determine the number of cells after 5 hours given an initial cell count and a specific generation time, we can use the formula for exponential growth: Nt = N0 * 2ⁿ. Here, N0 is the initial number of cells, n is the number of generations, and Nt is the final number of cells.
The generation time is 60 minutes (1 hour), meaning the cells double every hour. In 5 hours, the number of generations (n) is 5.
Start with an initial count of 3 cells: N0 = 3Calculate the final number of cells: Nt = 3 * 2⁵ = 3 * 32 = 96Therefore, after 5 hours, there will be 96 cells in the culture medium.
Complete question :
Three cells with generation times of 60 minutes are inoculated into a culture medium. how many cells are there after 5 hours?
A. 15
B. 180
C. 900
D. 32
E. 96
You pinch a patient's armpit in order to determine the patient's level of consciousness. the patient responds by moving away from the stimulus. how do you describe this patient's level of consciousness?
Cancerous cells may continuously change their glycocalyx. this may result in
The dri committee recommends that adults aged 51 years and older obtain 2.4 micrograms of vitamin b12 daily and that vitamin b12-fortified foods (such as fortified cereals) or supplements be used to meet much of the dri recommended intake. refer back to the information you obtained in a previous question about mrs. wylie. she be placed on a vitamin b12 supplement because
The method of heat transfer by which the sun warms the earth is
A.conduction
B. Convection
C. Radiation
D. Solaration
Answer:
C
Explanation:
Because between the earth and sun is empty space (vacuum) transfer of heat from the sun to earth can only occur through radiation- because convection and conduction require a medium. The is the same process that warms your hands when you place them beside a fire. When you put your hands above the fire, they are warmed through convection.
Prokaryotic organisms have recently been divided into two domains, bacteria and archaea. this division is based on characteristics such as
a.differences in cell wall composition.
b.differences in their initiator amino acid for start of protein synthesis.
c.presence or absence of histones.
d.b and c only
e.a, b, and c